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Y0771

Sigma-Aldrich

Anti-phospho-YAP1 (pTyr357) antibody, Mouse monoclonal

clone PYP-76, purified from hybridoma cell culture

Synonym(s):

Anti-COB1, Anti-YAP, Anti-YAP-1, Anti-YAP2, Anti-YAP65, Anti-YKI

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.44

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

PYP-76, monoclonal

form

buffered aqueous solution

mol wt

antigen ~65 kDa

species reactivity

human

technique(s)

indirect ELISA: suitable
indirect immunofluorescence: suitable
western blot: 0.5-1 μg/mL using total cell extract of HEK-293T cells co-transfected with wt YAP and a constitutively active mutant of c-Abl (Δ1-81)

isotype

IgM

UniProt accession no.

P46937

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pTyr357)

Gene Information

human ... YAP1(10413)
mouse ... Yap1(22601)

General description

Monoclonal Anti-phospho-YAP1 (pTyr357) (mouse IgM isotype) is derived from the hybridoma PYP-76 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic phosphopeptide corresponding to a fragment containing (pTyr357) of human YAP1 conjugated to KLH. Yes-associated protein 1 (YAP1) is a modular adapter protein with multiple protein interaction domains that was originally identified based on its interaction with the Src-family tyrosine kinase c-Yes. YAP1 contains a proline-rich amino terminus, a WW domain, a coiled-coil and a PDZ-binding motif at the extreme C-terminus.

Application

Anti-phospho-YAP1 (pTyr357) antibody, Mouse monoclonal has been used in
  • immunoblotting
  • enzyme linked immunosorbent assay (ELISA)
  • immunofluorescence

Biochem/physiol Actions

YAP1 has been shown to stabilize p73 by preventing Itch-mediated ubiquitination of p73.
YAP1 is a WW domain containing protein that is known to associate with a wide range of transcription factors and functions as a transcriptional co-activator. It may also regulate p73 gene function in response to DNA damage. Alterations in YAP1 have been associated with tumor development

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Conjugated Bile Acids Promote Invasive Growth of Esophageal Adenocarcinoma Cells and Cancer Stem Cell Expansion via Sphingosine 1-Phosphate Receptor 2-Mediated Yes-Associated Protein Activation
Liu R, et al.
The American Journal of Pathology, 188(9), 2042-2058 (2018)
Ting-Fang Lee et al.
Oncotarget, 8(52), 89539-89551 (2017-11-23)
Epidermal growth factor receptor (EGFR) mutations are found in lung adenocarcinomas leading to tumor cells proliferation and survival. EGFR tyrosine kinase inhibitors (TKIs) that block EGFR activity are effective therapeutics for EGFR-mutant lung adenocarcinoma patients, but TKI-resistance inevitably occurs. The
The Yes-associated protein 1 stabilizes p73 by preventing Itch-mediated ubiquitination of p73
Levy D, et al.
Cell Death and Differentiation, 14(4), 743-743 (2007)
Akihiko Komuro et al.
The Journal of biological chemistry, 278(35), 33334-33341 (2003-06-17)
The ErbB-4 receptor protein-tyrosine kinase is proteolytically processed by membrane proteases in response to the ligand or 12-O-tetradecanoylphorbol-13-acetate stimulation resulting in the cytoplasmic fragment translocating to the cell nucleus. The WW domain-containing co-transcriptional activator Yes-associated protein (YAP) associates physically with
D Levy et al.
Cell death and differentiation, 14(4), 743-751 (2006-11-18)
Upon DNA damage signaling, p73, a member of the p53 tumor suppressor family, accumulates to support transcription of downstream apoptotic genes. p73 interacts with Yes-associated protein 1 (Yap1) through its PPPY motif, and increases p73 transactivation of apoptotic genes. The
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