X1629

Anti-XPG (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-COFS3, Anti-ERCC5, Anti-ERCM2, Anti-UVDR, Anti-XPGC
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: human
• concentration: ~1 mg/mL
• technique(s): indirect immunofluorescence: 2-4 μg/mL using HEK-293T cells expressing recombinant XPG fusion protein, fixed with paraformaldehyde-Triton; western blot: 1-2 μg/mL using whole cell lysates of COS7 cells expressing recombinant XPG fusion protein
• UniProt accession no.: P28715
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ERCC5(2073)

Description

General description

Anti-XPG (C-terminal) is produced in rabbit using as immunogen a synthetic peptide corresponding to amino acids 1120-1137 of human XPG, conjugated to KLH via an N-terminal added cysteine residue. Xeroderma pigmentosum group G (XPG) is a DNA repair gene, that is mapped to human chromosome13q33. It is a member of the FEN-1 family of structure-specific nucleases and contains two highly conserved nuclease motifs known as the N and I regions separated by a large insertion. This gene spans around 69kb in length and has15 exons. It is highly polymorphic.

Immunogen

synthetic peptide corresponding to amino acids 1120-1137 of human XPG, conjugated to KLH via an N-terminal added cysteine residue.

Application

Anti-XPG (C-terminal) antibody produced in rabbit has been used in:

• staining cells for xeroderma pigmentosum group G (XPG) detection
• immunoblotting
• immunofluorescence

Biochem/physiol Actions

Xeroderma pigmentosum group G (XPG) helps to maintain genomic stability. It also participates in RNA transcription by interacting with TFIIH, RNA polymerase II and Gadd45a. XPG also participates in nucleotide excision repair (NER) pathway. Mutations in the XPG gene cause the Cockayne syndrome which is characterized by severe growth defects, mental retardation and cachexia.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)