U7883

Monoclonal Anti-USP25 antibody produced in mouse

~1.0 mg/mL, clone U25.7, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-USP21, Anti-Ubiquitin specific peptidase 25
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: U25.7, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~126 kDa
• species reactivity: rat, mouse, human
• concentration: ~1.0 mg/mL
• technique(s): immunocytochemistry: suitable; indirect ELISA: suitable; western blot: 1-2 μg/mL using total extract of HEK-293T cells expressing USP25.
• isotype: IgG2a
• UniProt accession no.: Q9UHP3
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... USP25(29761) ; mouse ... Usp25(30940) ; rat ... Usp25(304150)

Description

General description

Monoclonal Anti-USP25 (mouse IgG2a isotype) is derived from the hybridoma U25.7 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human USP25. USP25 (ubiquitin-specific protease 25) is a member of the ubiquitin processing proteases (UBP) family. It is located on the gene-poor region of 21q11.2 human chromosome.

Application

Monoclonal Anti-USP25 antibody has been used in:

• enzyme linked immunosorbent assay (ELISA)
• immunoblotting
• immunocytochemistry

Biochem/physiol Actions

USP25 (ubiquitin-specific protease 25) is a target for sumoylation. It takes place within the two ubiquitin interaction motifs (UIM) of USP25 that are required for efficient hydrolysis of Ub (ubiquitin), and thus impairs USP25 activity as a result of reduced affinity to Ub chains. Overexpression of USP25 has been observed in Down syndrome fetal brain.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)