TGEN-CNP
Cas9 Nickase Recombinant protein from Streptococcus pyogenes
lyophilized powder
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.51
form
lyophilized powder
Quality Level
packaging
pkg of 250 μg
pkg of 50 μg
shipped in
ambient
storage temp.
−70°C
General description
Recombinant Cas9 proteins (∼160 KD) are ready-to-use reagents for genome engineering experiments.
When combined with target-specific guide RNAs, Cas9 proteins will form ready-to-use nucleases suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection.
When combined with target-specific guide RNAs, Cas9 proteins will form ready-to-use nucleases suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection.
Features and Benefits
- Highly Specific
- Highly Active
- Ready-to-inject/transfect
Packaging
The kit comes in two pack sizes:
- TGEN-CNP-50UG
- TGEN-CNP-250UG
Components
Each kit consists of:
- one vial of Cas9 recombinant protein
- one vial containing 1 mL of 1x dilution buffer
- one vial containing 1mL of Nuclease free water with glycerol
Principle
CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids.
Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.
Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.
Linkage
Use our CRISPR Selection Tool to order gRNA atsigma.com/crisprs
Know more about Cas9 Nickase Recombinant protein
Know more about Cas9 Nickase Recombinant protein
Physical form
Lyophilized Recombinant_Sp_Cas9 protein
Preparation Note
It is recommended that the lyophilized Cas9 protein be resuspended in the nuclease-free water containing 50% glycerol that is provided in the kit to get the desired
concentration.
The solution should be allowed to incubate at room temperature (20−23 °C) for 10 minutes. Centrifuge immediately before use.
concentration.
The solution should be allowed to incubate at room temperature (20−23 °C) for 10 minutes. Centrifuge immediately before use.
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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