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T5694

Sigma-Aldrich

TRAIL human

recombinant, expressed in NSO cells, >97% (SDS-PAGE), lyophilized powder

Synonym(s):

Apo2 Ligand, Apo2L, TL2, TNF-related apoptosis-inducing ligand

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About This Item

CAS Number:
2244730-93-4
MDL number:
MFCD01867158
UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

Quality Level

200

recombinant

expressed in NSO cells

description

N-terminal 6X histidine-tagged extracellular domain

assay

>97% (SDS-PAGE)

form

lyophilized powder

mol wt

24 kDa by SDS-PAGE
predicted mol wt ~21 kDa

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

P50591

storage temp.

−20°C

Gene Information

human ... TNFSF10(8743)

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Application

TRAIL human was used to induce cytotoxicity in A549, a human lung carcinoma cell line.

Biochem/physiol Actions

TNF-related apoptosis-inducing ligand (TRAIL) is a type II transmembrane protein that belongs to TNF family. It is present in both soluble as well as membrane-bound form and can induce rapid apoptosis of various transformed cell lines but not the normal cells. Additionally, the product can also induces apoptotic cell death in TRAIL-sensitive U343MGcells.
Type II transmembrane protein of the TNF superfamily. Ligand for apoptosis-inducing death receptors DR4 and DR5, decoy receptors DcR1 and DcR2, and osteoprotegerin. Approximately 28% amino acid sequence identity to FAS ligand. Human and mouse TRAIL share 65% sequence identity.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

Analysis Note

Induces cytotoxicity in mouse L-929 cells in the presence of actinomycin D.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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J Sträter et al.
Annals of the New York Academy of Sciences, 915, 162-170 (2001-02-24)
The tumor necrosis factor receptor (TNFR) family is a still-growing group of homologous transmembrane proteins, some of which bear an intracellular "death domain" and are able to directly mediate apoptosis. Apoptosis is induced upon trimerization of the receptors by their
Matthew J Coussens et al.
Journal of visualized experiments : JoVE, (58)(58), doi:10-doi:10 (2012-01-05)
RNA interference (RNAi) is an intrinsic cellular mechanism for the regulation of gene expression. Harnessing the innate power of this system enables us to knockdown gene expression levels in loss of gene function studies. There are two main methods for
W S Simonet et al.
Cell, 89(2), 309-319 (1997-04-18)
A novel secreted glycoprotein that regulates bone resorption has been identified. The protein, termed Osteoprotegerin (OPG), is a novel member of the TNF receptor superfamily. In vivo, hepatic expression of OPG in transgenic mice results in a profound yet nonlethal
X D Zhang et al.
FEBS letters, 482(3), 193-199 (2000-10-12)
Resistance of normal cells to tumour necrosis factor related apoptosis inducing ligand (TRAIL) induced apoptosis is believed to be mediated by expression of two decoy receptors. Here we show that the expression and localisation of TRAIL receptors (TRAIL-Rs) vary between
Ling Xu et al.
Oncology reports, 32(1), 318-324 (2014-05-21)
Gastric cancer cells are insensitive to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), and we recently showed that lipid raft-regulated epidermal growth factor receptor (EGFR) activation antagonized TRAIL-induced apoptosis. However, it is not clear whether caveolin-1, an essential structural constituent of
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