SRP6217

Fetuin A/ASHG human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonym(s): Alpha-2-HS-glycoprotein, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in HEK 293 cells
• assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• mol wt: 45-55 kDa
• packaging: pkg of 10 and 50 μg
• impurities: <1 EU/μg endotoxin (LAL test)
• UniProt accession no.: P02765
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... FETUA(197)

Description

General description

Fetuin A/AHSG is a human plasma glycoprotein belonging to the Cystatin family of proteases inhibitors. It is highly expressed in embryonic cells and adult hepatocytes, and is expressed to a lesser extent in monocytes/macrophages. Fetuin A/AHSG is a major serum protein component that exerts various calcium dependent physiological activities, and can mediate growth signaling in certain tumor cells. It also can act as a natural antagonist against specific TGF-β and BMP signaling proteins. Recombinant human Fetuin-A is a heterodimeric protein containing a 282 amino acid A chain and a 27 amino acid B chain. Due to glycosylation, recombinant Fetuin-A migrates at an apparent molecular weight of 45 – 55 kDa by SDS-PAGE run under non-reducing conditions.

Physical form

Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 7.8.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable