All Photos(1)

Key Documents

SRP3244

IL-7 from rat

Name: IL-7 from rat
Brand: SIGMA

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):

Lymphopoietin 1(LP-1), pre-B cell factor

Sign Into View Organizational & Contract Pricing

All Photos(1)

About This Item

UNSPSC Code:

12352202

NACRES:

NA.32

biological source

rat

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

≤0.2 ng/mL ED₅₀

mol wt

15.0 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

P56478

shipped in

wet ice

storage temp.

−20°C

Gene Information

rat ... IL7(25647)

General description

IL-7 is a hematopoietic growth factor, which affects primarily early B and T cells. Produced by thymic stromal cells, spleen cells and keratinocytes, IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors such as ConA and IL-2. Human and murine IL-7 is cross-species reactive. Recombinant rat IL-7 is a 15.0 kDa protein containing 130 amino acid residues.

Biochem/physiol Actions

IL-7 is a hematopoietic growth factor, which affects primarily early B and T cells. Recombinant rat IL-7 is a 15.0 kDa protein containing 130 amino acid residues.

Sequence

MDCHIKDKDG KAFGSVLMIS INQLDKMTGT DSDCPNNEPN FFKKHLCDDT KEAAFLNRAA RKLRQFLKMN ISEEFNDHLL RVSDGTQTLV NCTSKEEKTI KEQKKNDPCF LKRLLREIKT CWNKILKGSI

Physical form

Lyophilized from 10 mM Sodium Acetate, pH 5.8.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Spits, H.

Cytokine Reference, 137-153 (2001)

B cell precursor growth-promoting activity. Purification and characterization of a growth factor active on lymphocyte precursors.

A E Namen et al.

The Journal of experimental medicine, 167(3), 988-1002 (1988-03-01)

We have used a biological assay system we developed to biochemically purify a previously uncharacterized murine lymphopoietic growth factor designated lymphopoietin 1 (LP-1). This factor is capable of stimulating the proliferation and extended maintenance of precursor cells of the B

Designing the epitope flanking regions for optimal generation of CTL epitopes.

Nicholas J Steers et al.

Vaccine, 32(28), 3509-3516 (2014-05-06)

The flanking amino acids that surround epitopes are critical for effective antigen processing and maintenance of epitope integrity. In the present study, the frequency and characteristics of each amino acid that flanked the peptides generated from the proteasomal degradation of

Combined ART started during acute HIV infection protects central memory CD4+ T cells and can induce remission.

Antoine Chéret et al.

The Journal of antimicrobial chemotherapy, 70(7), 2108-2120 (2015-04-23)

Therapeutic control of HIV replication reduces the size of the viral reservoir, particularly among central memory CD4+ T cells, and this effect might be accentuated by early treatment. We examined the effect of ART initiated at the time of the

Analysis of the functional WT1-specific T-cell repertoire in healthy donors reveals a discrepancy between CD4(+) and CD8(+) memory formation.

Sabine Schmied et al.

Immunology, 145(4), 558-569 (2015-04-18)

The Wilms' tumour-1 (WT1) protein is considered a prime target for cancer immunotherapy based on its presumptive immunogenicity and widespread expression across a variety of malignancies. However, little is known about the naturally occurring WT1-specific T-cell repertoire because self-derived antigens

View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service