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Key Documents

SRP2078

Sigma-Aldrich

p53 (1-342) C-terminal deletion human

recombinant, expressed in insect cells, ≥80% (SDS-PAGE)

Synonym(s):

FLJ92943, LFS1, P53, TRP53

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.26

biological source

human

recombinant

expressed in insect cells

assay

≥80% (SDS-PAGE)

form

frozen liquid

mol wt

~39.5 kDa

packaging

pkg of 5 μg

concentration

250 μg/mL

color

clear colorless

NCBI accession no.

NM_000546

UniProt accession no.

P04637

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... TP53(7157)

Biochem/physiol Actions

Human p53 protein is composed of 393 amino acid residues with several distinct regions. The N-terminal activation domain allows p53 protein to recruit the basal transcription machinery and activate the expression of target genes. The core domain binds to target DNA in a sequence-specific manner and the majority of mutations found in human tumors occur in the region of the gene encoding this domain. The C-terminal domain is composed of predominantly basic residues and modification of the C-terminal basic domain, including acetylation, glycosylation and phosphorylation, is an essential mechanism for regulating p53 function. Disruption or loss of oligomerization function is associated with loss of cell cycle arrest. This mutant protein (with the deletion of the C-terminus 51 residues including the entire basic domain and a portion of the tetramerization domain) can be used as a unique tool to study specific functions of p53 related to the C-terminus.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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X Chen et al.
Genes & development, 10(19), 2438-2451 (1996-10-01)
It is well established that induction of the p53 tumor suppressor protein in cells can lead to either cell cycle arrest or apoptosis. To further understand features of p53 that contribute to these cell responses several p53-null Saos2 and H1299
W S el-Deiry et al.
Nature genetics, 1(1), 45-49 (1992-04-01)
Recent experiments have suggested that p53 action may be mediated through its interaction with DNA. We have now identified 18 human genomic clones that bind to p53 in vitro. Precise mapping of the binding sequences within these clones revealed a
M Hollstein et al.
Science (New York, N.Y.), 253(5015), 49-53 (1991-07-05)
Mutations in the evolutionarily conserved codons of the p53 tumor suppressor gene are common in diverse types of human cancer. The p53 mutational spectrum differs among cancers of the colon, lung, esophagus, breast, liver, brain, reticuloendothelial tissues, and hemopoietic tissues.

Articles

Oncogenes and Tumor Suppressors Reprogram Metabolism

We present an article about how proliferating cells require the biosynthesis of structural components for biomass production and for genomic replication.

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