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Key Documents

SRP0373

Sigma-Aldrich

CDK4/Cyclin D1 active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥90% (SDS-PAGE)

Synonym(s):

BCL1, CCND1, Cell division protein kinase 4, Cyclin-dependent kinase 4, PRAD1, PSK-J3

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected Sf9 cells

assay

≥90% (SDS-PAGE)

form

aqueous solution

mol wt

29 kDa (Cyclin D1)
 34 kDa (CDK4)

packaging

pkg of 20 μg

solubility

water: soluble

NCBI accession no.

NM_000075 (CDK4)
NM_053056 (cyclin D1)

application(s)

life science and biopharma

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CCND1(595) , CDK4(1019)

General description

Research area: Apoptosis

Cyclin D1 (CCND1) is a proto-oncogene. It is one of the three D-type cyclins. The gene encoding it is localized on human chromosome 11q13. Cyclin dependent kinase-like 4 (CDKL4) is part of the cyclin-dependent protein kinase (CDK) family. The gene encoding it is localized on human chromosome 12q14-15.
Recombinant, full length human CDK4 (Genbank Accession No. NM_000075), amino acids 16-271, with C-terminal His-tag and modifications (amino acids 43G-47G were replaced with EE, and T172A), MW=34 kDa, and human Cyclin D1 (Genbank accession number NM_053056), MW 29 kDa, were co-expressed in a Baculovirus-infected Sf9 cells expression system.

Biochem/physiol Actions

Cyclin D1 (CCND1) regulates the Wnt signaling pathway. It also regulates the progression of the cell cycle from the G1 to S phase in many cell types. CCND1 phosphorylates and inactivates retinoblastoma (Rb) protein. Cyclin D1 has been associated with mantle cell lymphoma. This protein is overexpressed in multiple cancers like hepatocellular carcinoma. Cyclin dependent kinase-like 4 (CDKL4) has been associated with various tumors and colorectal cancer. The protein regulates apoptosis of nucleated cells by modifying transcriptional regulation of genes governing cell proliferation and cell death. Like CCND1, it also modulates the progression of cycle from G1 to S phase.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Association Between Polymorphism rs678653 in Human Cyclin D1 Gene (CCND1) and Susceptibility to Cancer: A Meta-Analysis
Xichao Dai
Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 22, 863-874 (2016)
miR-16 induction after CDK4 knockdown is mediated by c-Myc suppression and inhibits cell growth as well as sensitizes nasopharyngeal carcinoma cells to chemotherapy.
Jiang Q
Tumour Biology : the Journal of the International Society For Oncodevelopmental Biology and Medicine, 37(2), 2425-2433 (2016)
Impact of cyclin-dependent kinase CDK4 inhibition on eryptosis.
Lang E
Cellular Physiology and Biochemistry, 37(3), 1178-1186 (2015)
Gains and complex rearrangements of the 12q13-15 chromosomal region in ordinary lipomas: The ?missing link? between lipomas and liposarcomas?
Antoine Italiano
Cancer Genetics, 121(2), 308-315 (2007)
Cyclin D1 expression and polysomy in lymphocyte-predominant cells of nodular lymphocyte-predominant Hodgkin lymphoma
Benjamin B. Cho
Annals of diagnostic pathology, 26 (2016)
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