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Key Documents

SRP0353

Sigma-Aldrich

Akt1 active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥75% (SDS-PAGE)

Synonym(s):

PKB, RAC, v-akt murine thymoma viral oncogene homolog 1

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected Sf9 cells

assay

≥75% (SDS-PAGE)

form

aqueous solution

mol wt

60.2 kDa

packaging

pkg of 10 μg

NCBI accession no.

NM_001014432

UniProt accession no.

P31749

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... AKT1(207)

General description

AKT1 (v-akt murine thymoma viral oncogene homolog 1) is a Ser/Thr protein kinase, and is also called protein kinase B (PKB). This protein is activated when it is phosphorylated at serine 473 and threonine 308. This gene is localized to human chromosome 14q32.32.

Application

Akt1 active human has been used for the testing of recombinant mouse p21 proteins as its substrates using in vitro protein kinase assay.

Biochem/physiol Actions

AKT1 (v-akt murine thymoma viral oncogene homolog 1) is responsible for the phosphorylation and cytoplasmic localization of CKDN1A (cyclin-dependent kinase (CDK) inhibitor 1, also referred to as p21). However, p21 is one of the CDKNs which normally resides in the nucleus and its cytoplasmic localization, mediated by AKT1, is common to various cancers where it suppresses proteins essential for apoptosis. Studies show that up-regulation of AKT1 serves as a poor prognostic marker in breast cancer, which however, depends upon factors including population, hormone receptor status, and hormonal or trastuzumab therapy status.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Vogiatzi P and Giordano A.
Cancer Biology & Therapy, 6 (2007)
Coenraad Kuijl et al.
Nature, 450(7170), 725-730 (2007-11-30)
With the emergence of multidrug resistant (MDR) bacteria, it is imperative to develop new intervention strategies. Current antibiotics typically target pathogen rather than host-specific biochemical pathways. Here we have developed kinase inhibitors that prevent intracellular growth of unrelated pathogens such
Edgar Gil Rizzatti et al.
British journal of haematology, 130(4), 516-526 (2005-08-16)
Microarray studies have revealed the differential expression of several genes in mantle cell lymphoma (MCL), but it is unknown which of these differences are dependent on the transformed MCL cell itself or on the tumour microenvironment. To investigate which genes
Zu-Yao Yang et al.
Scientific reports, 5, 7758-7758 (2015-01-15)
The prognostic value of phosphorylated Akt (pAkt) overexpression in breast cancer has been investigated by many studies with inconsistent results. This systematic review was conducted to evaluate the association of pAkt overexpression with breast cancer prognosis in terms of overall
Christelle de Renty et al.
PloS one, 9(5), e97434-e97434 (2014-05-23)
Proliferating trophoblast stem cells (TSCs) can differentiate into nonproliferating but viable trophoblast giant cells (TGCs) that are resistant to DNA damage induced apoptosis. Differentiation is associated with selective up-regulation of the Cip/Kip cyclin-dependent kinase inhibitors p57 and p21; expression of
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