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Key Documents

SRP0187

Sigma-Aldrich

Bcl-xL Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonym(s):

Apoptosis regulator Bcl-X, BCL-XL/S, BCL2L1, Bcl-2-like protein 1

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

assay

≥90% (SDS-PAGE)

form

aqueous solution

mol wt

28 kDa

packaging

pkg of 100 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

Z23115

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... BCL2L1(598)

General description

BCL2L1 (B-cell lymphoma 2 like 1) gene codes for the anti-apoptotic protein called Bcl-xL (B-cell lymphoma-extra large) and its isoform Bcl-xS. Bcl-xS functions as an antagonist of Bcl-xL. This gene is localized to human chromosome 20q11.21.

Application

Useful for the study of apoptosis, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

BCL2L1 (B-cell lymphoma 2 like 1) or Bcl-xS functions as a dominant negative regulator of the anti-apoptotic isoform Bcl-xL. Bcl-xL is involved in modulating anti-apoptotic capacity of chemotherapy-resistant tumors. This protein is highly expressed in michigan cancer foundation 7 (MCF7) human breast carcinoma cells, and studies show that overexpression of Bcl-xS in these MCF-7 cells results in heightened sensitivity to chemotherapy-induced apoptosis by agents such as VP-16 or taxol.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 2

Certificates of Analysis (COA)

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Overexpression of Bcl-XS sensitizes MCF-7 cells to chemotherapy-induced apoptosis.
Sumantran VN, et al.
Cancer Research, 55(12), 2507-2510 (1995)
Gain of 20q11.21 in human embryonic stem cells improves cell survival by increased expression of Bcl-xL.
Nguyen HT, et al.
Molecular Human Reproduction, 20(2), 168-177 (2014)
Bcl-xL mediates therapeutic resistance of a mesenchymal breast cancer cell subpopulation.
Keitel U, et al.
Oncotarget, 5(23), 11778-11791 (2014)
BCL-XL mediates the strong selective advantage of a 20q11.21 amplification commonly found in human embryonic stem cell cultures.
Avery S, et al.
Stem Cell Reports, 1(5), 379-386 (2013)
Pawel Licznerski et al.
Cell, 182(5), 1170-1185 (2020-08-17)
Loss of the gene (Fmr1) encoding Fragile X mental retardation protein (FMRP) causes increased mRNA translation and aberrant synaptic development. We find neurons of the Fmr1-/y mouse have a mitochondrial inner membrane leak contributing to a "leak metabolism." In human
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