SML3736

Hypoxia probe 2

95% (UPLC)

• Synonym(s): 5’-Methoxy-1’,2’-dimethyl-3-(1-((3-oxo-3H-phenoxazin-7-yl)oxy)ethyl)-1H-indole-4’,7’-dione
• Empirical Formula (Hill Notation): C₂₅H₂₀N₂O₆
• Molecular Weight: 444.44
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• assay: 95% (UPLC)
• form: powder
• storage condition: protect from light
• color: , Faint orange to red
• solubility: DMSO: 2 mg/mL, clear (Warmed)
• shipped in: wet ice
• storage temp.: -10 to -25°C

Description

Biochem/physiol Actions

Hypoxia probe 2 is a resorufin-based non-fluorescent hypoxia probe that undergoes cytochrome P450 reductase (PH51)-catalyzed reduction to yield the fluorescent reporter resorufin (ex 545 nm, em 582 nm) in conditions of 4% O2 and lower, allowing imaging hypoxia levels similar to those detected by pimonidazole without the use of antibodies. When employed together with Tokyo green-based hypoxia probe 4 (Tokyo green ex 480 nm, em 515 nm), the probe pair allows the simultaneous (two-color) imaging of different hypoxia levels in living cells.

Resorufin-based hypoxia probe that allows fluorescent imaging of hypoxia levels in live cells similar to those detected by pimonidazole without the use of antibodies.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable