SML2959

Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone Ready Made Solution

≥98% (HPLC), liquid, mithophagy inducer

• Synonym(s): FCCP, Mesoxalonitrile 4-trifluoromethoxyphenylhydrazone
• Empirical Formula (Hill Notation): C₁₀H₅F₃N₄O
• Molecular Weight: 254.17
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• product name: Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone Ready Made Solution, ≥98% (HPLC), 2.54 mg/mL (10mM) in DMSO
• Quality Level: 100
• assay: ≥98% (HPLC)
• form: liquid
• storage temp.: −20°C

Description

General description

Carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP) is a proton (H⁺) ionophore and a potent mitochondrial uncoupling agent. This compound turns the mitochondrial membrane permeable to protons, thus dissipating the mitochondrial membrane potential and uncouples oxidative phosphorylation from ATP synthesis. FCCP is a potent tool for assaying mitochondrial respiration and investigating the role of mitochondria in cellular function.^1,2FCCP induces mitophagy of SH-SY5Y neuroblastoma cell in a dose dependent manner. Low concentration of FCCP (1μM) leads to a complete loss of mitochondrial membrane potential without triggering mitophagy, whereas at a high concentration (10 μM), cytosol acidification is increased and mitochondrial degradation is attained.³ Additionally, FCCP (20 μM) was able to induce apoptosis in lymphoid (CCRF-CEM) and myeloid (HL60) cells after 24hrs of incubation. ⁴ FCCP (0.5nM-5 μM) reduces β-amyloid peptide production in K695sw cells without altering the β-amyloid precursor protein maturation or synthesis.⁵ Partial uncoupling of mitochondrial oxidative phosphorylation induced by FCCP (0.3 -3μM) reduces micromotion and wound healing migration of human mesenchymal stem cells.⁶ Inhibition of F1F0-ATPase by FCCP (10 μM), reduces the impairment of electromechanical activity in human myocardium.⁷

Biochem/physiol Actions

Mithophagy inducer, ionophore

Other Notes

FCCP ready made solution is a DMSO based solution at 10mM concentration. It is recommended to further dilute in a suitable media to achieve a working concentration of 1-10 μM.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: 188.6 °F
• flash_point_c: 87 °C