SBR00047

Rhodamine B labeled Ramoplanin

For fluorescent microbial imaging

• Empirical Formula (Hill Notation): C₁₄₆H₁₈₄ClN₂₃O₄₆S₂
• Molecular Weight: 3096.73
• UNSPSC Code: 12352116
• NACRES: NA.76

Properties

• Quality Level: 100
• form: solid
• specific activity: Antibiotic (bacterial staining)
• storage temp.: −20°C

Description

General description

Rhodamine B labeled Ramoplanin is a fluorescent derivative of Ramoplanin, with a similar mode of action as Ramoplanin. It allows fluorescent labeling of Gram-positive bacteria and their detection by common fluorescent instruments. Ramoplanin is a lipoglycodepsipeptide antibiotic that is produced by the fermentation of Actinoplanes species. Fluorescent antibiotics are obtained by a synthetic conjugation of an antibiotic to a fluorophore.

Application

Fluorescent antibiotics can be used for many applications including:

• Antimicrobial resistance research
• Bacterial visualization and imaging
• Parent antibiotic mode of action research and new antibiotic discovery
• Toxicity studies
• Diagnosis of bacterial infections and tracking their uptake in vivo

Biochem/physiol Actions

Ramoplanin blocks the bacterial cell wall biosynthesis by specifically binding to the first sugar of lipid I. N-Acetylmuramic acid thus interfering with peptidoglycan production.

Mode of Action: Is different from the d-Ala-d-Ala that are targeted by vancomycin and shows no cross-resistance with other glycopeptides.

Activity Spectrum: Effective against a broad-spectrum activity against Gram-positive pathogens both in vitro and in vivo including Enterococci, Staphylococci, Bacilli, Streptococci, Listeria monocytogenes, and Gram-positive anaerobes such as Clostridrium difficile. However, Rhodamine B labeled Ramoplanin presents a MIC decrease in the magnitude of two to five orders in bacterial activity in comparison to the parent Ramoplanin.

Features and Benefits

High quality antibiotic suitable for mulitple research applications

Analysis Note

• Fluorescent microscopy application: Rhodamine B labeled Ramoplanin has excitation/emission wavelength range at 550-560 nm/590-600 nm.
• It is recommended to dissolve the Rhodamine B labeled Ramoplanin in DMSO to a concentration of 2 mg/mL.
• The recommended working concentration in fluorescent microscopy imaging application is 10μM. The mentioned concentration was used for Bacillus subtills staining (see image).
• Aliquots of the DMSO solution can be stored at −20 °C, protected from light for at least one month.

Other Notes

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Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable