SAE0104
BmrA (Bacilus Subtilis Multidrug Resistance Abc Transporter Atp-Binding Protein)
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.83
Recommended Products
recombinant
expressed in E. coli
description
C-Terminal six-histidine tagged
assay
≥90% (SDS-PAGE)
form
aqueous solution
mol wt
64.5 kDa
concentration
0.3 mg/mL
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Biochem/physiol Actions
BmrA is an efflux protein and ABC transporter that acts as an ATP-dependent drug efflux pump.
Preparation Note
Formulated in 50mM Tris-Cl pH 8.0, 100mM NaCl, 0.01% DDM, 10% glycerol.
Storage and Stability
Thaw on ice. Upon first thaw, briefly spin tube containing protein to recover full content of the tube. Aliquot into single use aliquots. Store remaining undiluted protein in aliquots at -80°C. Note: avoid freeze/thaw cycles.
Substrates
MSSSHHHHHHMPTKKQKSKSKLKPFFALVRRTNPSYGKLAFALALSVVTTLVSLLIPLLTKQLVDGFSMSNLSGTQIGLIALVFFVQAGLSAYATYALNYNGQKIISGLRELLWKKLIKLPVSYFDTNASGETVSRVTNDTMVVKELITTHISGFITGIISVIGSLTILFIMNWKLTLLVLVVVPLAALILVPIGRKMFSISRETQDETARFTGLLNQILPEIRLVKASNAEDVEYGRGKMGISSLFKLGVREAKVQSLVGPLISLVLMAALVAVIGYGGMQVSSGELTAGALVAFILYLFQIIMPMGQITTFFTQLQKSIGATERMIEILAEEEEDTVTGKQIENAHLPIQLDRVSFGYKPDQLILKEVSAVIEAGKVTAIVGPSGGGKTTLFKLLERFYSPTAGTIRLGDEPVDTYSLESWREHIGYVSQESPLMSGTIRENICYGLERDVTDAEIEKAAEMAYALNFIKELPNQFDTEVGERGIMLSGGQRQRIAIARALLRNPSILMLDEATSSLDSQSEKSVQQALEVLMEGRTTIVIAHRLSTVVDADQLLFVEKGEITGRGTHHELMASHGLYRDFAEQQLKMNADLENKAG
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Emmanuelle Steinfels et al.
Biochemistry, 43(23), 7491-7502 (2004-06-09)
The involvement of transporters in multidrug resistance of bacteria is an increasingly challenging problem, and most of the pumps identified so far use the protonmotive gradient as the energy source. A new member of the ATP-binding cassette (ABC) family, known
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