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Key Documents

SAB5801020

Sigma-Aldrich

Alpaca Anti-Mouse IgM(µ chain) HRP single domain Antibody

recombinant Alpaca(VHH) Nano antibody

Synonym(s):

Recombinant Alpaca single variable domain secondary Nanobody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

alpaca

recombinant

expressed in E. coli

conjugate

horse radish peroxidase (HRP)

antibody form

affinity purified immunoglobulin

antibody product type

secondary antibodies

clone

recombinant monoclonal

form

lyophilized solid

mol wt

14 kDa

species reactivity

Alpaca, mouse

technique(s)

ELISA: 1:5000-1:20000
western blot: 1:5000-1:20000

size

0.1 mL

color

colorless

isotype

VHH

shipped in

wet ice

storage temp.

2-8°C

General description

Alpaca Anti-Mouse IgM(µ chain) HRP single domain Antibody is designed to specifically detect mouse IgM. It is a monovalent, single domain, recombinant antibody to mouse IgM(µ chain specific) coupled to HRP. Based on immunoelectrophoresis and/or ELISA, Alpaca Anti-Mouse IgM(µ chain) HRP single domain Antibody detects the µ chain of mouse IgM selectively but not with mouse, rabbit, human, cynomolgus, rat, goat IgG.

Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. IgM accounts for 5-10% of the immunoglobulin pool and is the predominant antibody in the primary immune response. Unlike IgG, IgM does not contain a hinge region but does contain an additional constant domain. The monomeric form IgM has a molecular weight of 180 KD. It is classically represented as a pentamer of the basic four chain structure held together by a J chain but can also exist in a hexameric form without the J chain and as a monomer on the surface of B-cells.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Specificity

Fc region of mouse IgM(µ chain specific)
Isotype: VHH domain of alpaca IgG2b/2c

Immunogen

Recombinant Alpaca single variable domain secondary nano antibody

Packaging

100 μg in Glass bottle

Physical form

Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose

Reconstitution

Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL

Storage and Stability

After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Protect from light. Stable for 12 months at -20°C

Other Notes

No cross-reactivity with mouse, rabbit, human, cynomolgus, rat, goat IgG Affinity

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pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317,H412

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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