SAB4702008

Monoclonal Anti-CD73 FITC produced in mouse

• Synonym(s): CALJA, E5NT, NT5E, NTE, ecto-5´nucleotidase
• UNSPSC Code: 12352203

Properties

• conjugate: FITC conjugate
• Quality Level: 100
• antibody product type: primary antibodies
• clone: AD2, monoclonal
• form: buffered aqueous solution
• species reactivity: ( Non-human primates), human
• technique(s): flow cytometry: suitable
• isotype: IgG1κ
• UniProt accession no.: P21589
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... NT5E(4907)

Description

General description

CD73 (ecto-5´-nucleotidase) is a 70 kDa glycoprotein anchored to the extracellular leaflet of the plasma membrane by GPI. This ecto-enzyme catalyzes dephosphorylation of AMP to adenosine. CD73 is expressed in various types of cells, such as epithelial, muscle, and endothelial cells, neutrophils, lymphocytes and fibroblasts. Inflammatory mediators support CD73 expression and its enzymatic activity, leading to the release of adenosine, which modulates inflammation through adenosine receptors. CD73 is expressed in a variety of lymphomas and leukemias, including ALL and CLL, whereas immunodeficient patients usually express low levels of this protein.

Specificity

The mouse monoclonal antibody AD2 recognizes CD73, a 70 kDa GPI-anchored 5´-nucleotidase expressed predominantly on the surface of T and B cell subsets, follicular dendritic cells and endothelial cells.

Immunogen

pre-B leukemia cells

Physical form

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preparation Note

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Storage and Stability

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Other Notes

The antibody solution should be gently mixed before use.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable