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Key Documents

SAB4700023

Sigma-Aldrich

Monoclonal Anti-CD147-APC antibody produced in mouse

clone MEM-M6/1, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-BSG, Anti-Basigin, Anti-Neurothelin

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

Allophycocyanin conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MEM-M6/1, monoclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

flow cytometry: suitable

isotype

IgG1

NCBI accession no.

NM_001728.2

UniProt accession no.

P35613

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... BSG(682)

Related Categories

General description

The antibody MEM-M6/1 recognizes an epitope in the N-terminal Ig domain (D1) of CD147 (Neurothelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells; it is not expressed by resting lymphocytes.

Immunogen

Protein A-CR purified soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the hinge region, CH2-and CH3 domain of human IgG1 (CD147Rg is secreted by transfectants as a dimer)

Application

The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 1e6 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests.

Features and Benefits

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Physical form

Solution in phosphate buffered saline containing 15 mM sodium azide and 0.2% high-grade protease free BSA as a stabilizing agent.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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C Koch et al.
International immunology, 11(5), 777-786 (1999-05-18)
CD147 is a broadly expressed cell surface glycoprotein of the Ig superfamily whose expression is up-regulated upon T cell activation. In order to elucidate a possible role of CD147 in T cell biology, we established 15 specific mAb. Seven distinct

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