SAB4504331
Anti-phospho-Akt (pSer473) antibody produced in rabbit
affinity isolated antibody
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 55 kDa
species reactivity
human, mouse, rat
concentration
~1 mg/mL
technique(s)
ELISA: 1:5000
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pSer473)
Gene Information
human ... AKT1(207)
rat ... Akt1(24185)
General description
AKT1 (AKT serine/threonine kinase 1) is also called as v-akt murine thymoma viral oncogene homolog 1 and PKBα (protein kinase B). It is expressed in liver, muscle and adipocytes. AKT1 gene is mapped to human chromosome 14q32. It is a member of AKT family that has an amino-terminal pleckstrin homology (PH) domain, a short α helical linker and a carboxyl-terminal kinase domain.
Immunogen
The antiserum was produced against synthesized peptide derived from human Akt around the phosphorylation site of Ser473.
Immunogen Range: 431-480
Immunogen Range: 431-480
Application
Anti-phospho-Akt (pSer473) antibody has been used in western blotting.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Biochem/physiol Actions
AKT1 (AKT serine/threonine kinase 1) controls the survival of cells and anti-apoptotic actions that attack the pathogenesis of several cancers , hence it plays a crucial role in tumorigenesis. In mice, AKT1 is essential for normal development.
Features and Benefits
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Physical form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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