SAB4200822
Anti-LC3C antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Autophagy-related protein LC3 C, Autophagy-related ubiquitin-like modifier LC3 C, MAP1 light chain 3-like protein 3, MAP1A/MAP1B LC3 C, MAP1A/MAP1B light chain 3 C, Microtubule-associated protein 1 light chain 3 gamma, Microtubule-associated proteins 1A/1B light chain 3C
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All Photos(3)
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
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biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
~16/18 kDa
species reactivity
human
packaging
antibody small pack of 25 μL
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
concentration
~1 mg/mL
technique(s)
immunoblotting: 1:2000-1:4000 using HEK-293T cells overexpressing human LC3C.
immunofluorescence: 1:400-1:800 using human HeLa cells treated with Bafilomycin A1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... MAP1LC3C(440738)
Related Categories
General description
At least 16 genes encoding for autopahgy (ATG) related proteins that are required for autophagosome formation were identified in yeast by genetic screens. For many of these genes, related homologs have been identified in mammals.The Microtubule-associated protein light chain 3 (MAP1LC3), the mammalian homolog of yeast Atg8/Apg8/Aut7, is essential for the formation of autophagosomes. In addition, three human orthologs of yeast Atg8, named LC3A (MAP1LC3A), LC3B (MAP1LC3B) and LC3C (MAP1LC3C), were identified. These three human isoforms show different expression patterns in human tissues.The LC3 protein exists in two forms, cytoplasmic LC3-I (18 kDa) and autophagosome membrane associated LC3-II (16 kDa). LC3-II is the result of LC3-I cleavage at the C-terminal Glycine by cysteine protease Atg4, which is further modified by the mammalian E1- and E2-like enzymes: Atg7 and Atg3. The amount of LC3-II correlates with the extent of autophagosome formation. Thus, LC3C can be used as an autophagosomal and mitophagy marker.
Microtubule-associated protein light chain 3 (MAP1LC3) is the mammalian homolog of yeast Atg8/Apg8/Aut7. Three human orthologs of yeast Atg8, named LC3A (MAP1LC3A), LC3B (MAP1LC3B) and LC3C (MAP1LC3C), were identified. These three human isoforms show different expression patterns in human tissues. The LC3 protein exists in two forms, cytoplasmic LC3-I (18 kDa) and autophagosome membrane associated LC3-II (16 kDa).
Specificity
Anti-LC3C antibody specifically recognizes human LC3C-I and/or LC3C-II and does not cross react with human LC3A or LC3B.
Immunogen
Synthetic peptide corresponding to human N-terminal region LC3C, conjugated to KLH
Application
Anti-LC3C antibody produced in rabbit has been used in immunoblotting and immunofluorescence.
Biochem/physiol Actions
MAP1LC3 is essential for the formation of autophagosomes. LC3C is used as an autophagy and mitophagy marker.
Macroautophagy, usually referred to as an autophagy, is a major pathway for bulk degradation of cytoplasmic constituents and organelles. In this process, portions of the cytoplasm are sequestered into double membrane vesicles, called autophagosomes, and subsequently delivered to the lysosome for degradation and recycling. Although autophagy is a constitutive cellular event, it is enhanced under certain conditions such as starvation, hormonal stimulation and drug treatments. Autophagy plays an essential role in cellular differentiation, cell death and aging and is required for normal turnover of cellular components during starvation. Defective autophagy process may contribute to certain human diseases such as cancer, neurodegenerative diseases, muscular disorders and pathogen infections.Autophagy pathway is evolutionary conserved in all eukaryotic cells.
Physical form
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
Storage and Stability
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Takahiro Shintani et al.
Science (New York, N.Y.), 306(5698), 990-995 (2004-11-06)
Autophagy, the process by which cells recycle cytoplasm and dispose of excess or defective organelles, has entered the research spotlight largely owing to the discovery of the protein components that drive this process. Identifying the autophagy genes in yeast and
Akiko Kuma et al.
Nature, 432(7020), 1032-1036 (2004-11-05)
At birth the trans-placental nutrient supply is suddenly interrupted, and neonates face severe starvation until supply can be restored through milk nutrients. Here, we show that neonates adapt to this adverse circumstance by inducing autophagy. Autophagy is the primary means
Autophagy in the eukaryotic cell.
Fulvio Reggiori et al.
Eukaryotic cell, 1(1), 11-21 (2002-11-29)
Visualizing the autophagy pathway in avian cells and its application to studying infectious bronchitis virus
Maier HJ, et al.
Autophagy, 9(4), 496-509 (2013)
LC3, GABARAP and GATE16 localize to autophagosomal membrane depending on form-II formation
Kabeya Y, et al.
Journal of Cell Science, 117(13), 2805-2812 (2004)
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