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SAB4200741

Sigma-Aldrich

Anti-Human IgE antibody, Mouse monoclonal

clone GE-1, purified from hybridoma cell culture

Synonym(s):

Anti-Immunoglubulin E

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

Quality Level

200

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

GE-1, monoclonal

form

buffered aqueous solution

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

ELISA: 0.15-0.3 μg/mL using 1 μg/mL Human IgE myeloma for coating
immunoblotting: suitable
immunoprecipitation (IP): 1-2 μg/test using Human IgE myeloma

isotype

IgG2b

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Anti-Human IgE antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the GE-1 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with purified human IgE myeloma protein. Immunoglobulin E (IgE) is the least abundant immunoglobulin isotype in circulation. IgE is produced by plasma cells and is highly functional in the lung, skin and mucus membranes. Normal levels of IgE in serum are less than 1 μg/ml.

Immunogen

purified human IgE myeloma protein

Application

Anti-Human IgE antibody, Mouse monoclonal has been used in various immunological techniques, including enzyme linked immunosorbent assay (ELISA), immunoblot, immunoprecipitation and IgE quantification using piezoelectric (Pz) crystal bioassay.

Biochem/physiol Actions

Immunoglobulin E (IgE) plays a critical role in allergic reactions and host protection from parasites. Serum IgE levels are highly elevated during an allergic reaction (such as atopic eczema, extrinsic asthma or hay fever, fungal and parasitic infection, atopic disease or primary immunodeficiency). Detection and measurement of total IgE concentrations is crucial for diagnosis, monitoring and treatment of allergy patients. In addition, the recognition and isolation of allergen-specific IgE can lead to progress in understanding of pathophysiologic mechanisms underlying IgE-associated allergic disorders.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
017M4834V
51161203

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Immunoglobulin E in health and disease
Amarasekera M, et al.
Asia Pacific Allergy, 1(1), 12-15 (2011)
Allergic and nonallergic forms of atopic diseases
Novak N and Bieber T
The Journal of Allergy and Clinical Immunology, 112(2), 252-262 (2003)
Precision medicine allergy immunoassay methods for assessing immunoglobulin E sensitization to aeroallergen molecules
Popescu FD, et al.
World journal of methodology, 8, 17-17 (2018)
Factors affecting human IgE and IgG responses to allergen-like Schistosoma mansoni antigens: Molecular structure and patterns of in vivo exposure
Fitzsimmons CM, et al.
International Archives of Allergy and Immunology, 142, 40-50 (2019)
R H Buckley et al.
The Journal of clinical investigation, 55(1), 157-165 (1975-01-01)
Concentrations of IgD and IgE were measured in sera from 165 patients with well-defined immunodeficiency in an effort to find information possibly relevant to the roles of antibodies of these classes in host defense. Values for both immunoglobulins were generally
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