SAB4200739

Anti-Protein kinase C antibody, Mouse monoclonal

clone MC5, purified from hybridoma cell culture

• Synonym(s): Anti-PKC
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: MC5, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~80 kDa
• species reactivity: chicken, human, fish, mouse, bovine, rabbit, rat
• concentration: ~1.0 mg/mL
• technique(s): immunoblotting: 1.25-2.5 μg/mL using human bone marrow neuroblast SH-SY5Y cell extract; immunocytochemistry: suitable; immunofluorescence: suitable; immunoprecipitation (IP): suitable
• isotype: IgG2a
• UniProt accession no.: P04409
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PRKCA(5578)

Description

General description

Anti-Protein kinase C antibody, Mouse monoclonal (mouse IgG2a isotype) is derived from the MC5 hybridoma produced by the fusion of X-63 myeloma cells and splenocytes from a BALB/c mouse immunized with purified bovine brain protein kinase C1. Protein kinase C (PKC), is a family of phospholipid- and calcium-activated, diacylglycerol (DAG)-dependent serine/threonine kinases.

Specificity

Monoclonal Anti-Protein kinase C antibody recognizes an epitope located within the amino acid residues 296- 317, at the hinge region, close to or at the trypsin cleavage site of Protein kinase C molecule . The antibody specifically recognizes Protein kinase C from human, rat, bovine, mouse, chicken, rabbit and fish origin.

Application

Anti-Protein kinase C antibody has been used in:

• immunoblotting
• immunohistochemistry
• immunofluorescence
• immunoprecipitation

Biochem/physiol Actions

Protein kinase C (PKC) play a central role in a variety of cell processes, including: intracellular signal transduction, cell proliferation, apoptosis, differentiation, migration and adhesion, tumor promotion, cardiac hypertrophy, angiogenesis, platelet function, inflammation and oncogenesis. Neuronal PKC modulates several functions such as nerve cell excitability, neuronal plasticity and release of neurotransmitters. PKC activity is regulated by two mechanisms- firstly by phosphorylation which regulates the active site and subcellular localization of the enzyme, secondly by secondary messengers which facilitate association of PKC on membrane. The PKC activity is strongly associated with tumor initiation and progression and have been implemented as a target for treatment of cancer progression and diabetic retinopathy.

Physical form

Purified bovine brain protein kinase C

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20°C. For continuous use, store at 2–8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable