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Key Documents

SAB4200552

Sigma-Aldrich

Anti-WASH1 antibody, Mouse monoclonal

clone WASH1-27, purified from hybridoma cell culture

Synonym(s):

Anti-CXYorf1-like protein on chromosome 9, Anti-FAM39E, Anti-WAS protein family homolog 1, Anti-WASH, Monoclonal Anti-WASH1 antibody produced in mouse

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

WASH1-27, monoclonal

form

buffered aqueous solution

mol wt

antigen ~72 kDa

species reactivity

mouse, human

concentration

~1 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using lysates of human HeLa cells
western blot: 2.5-5.0 μg/mL using whole extracts of HEK-293T cells overexpressing mouse WASH1

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

General description

Monoclonal Anti-WASH1 (mouse IgG1 isotype) is derived from the hybridoma WASH1-27 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal region of human WASH1. WASH1 (Wiskott-Aldrich Syndrome Protein and SCAR Homolog), a new member of the Wiskott−Aldrich Syndrome protein (WASP) family. Similar to other WASP family members, it contains a C-terminal for ′WH2, connecting and acidic′ (WCA) domain that binds to actin and the Arp2/3 complex. In addition, WASH1 also contains a short proline-rich region, a unique N-terminal domain termed WASH-homology domain (WAHD1), and a tubulin-binding region. Through its WAHD1 region, it interacts with family with sequence similarity 21 (FAM21), a protein that links WASH1 to endosomes. WASH1 forms part of a multiprotein complex composed of FAM21, KIAA1033 strumpellin and WASH-interacting protein (SWIP) and coiled coil domain containing 53 (CCDC53).

Immunogen

a synthetic peptide corresponding to an internal region of human WASH1 (GeneID: 100287171), conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat.

Application

Monoclonal Anti-WASH1 antibody produced in mouse has been used in may be used in various immunochemical techniques including immunoblotting (~72 kDa), immunoprecipitation and immunofluorescence.

Biochem/physiol Actions

WASH1 (Wiskott-Aldrich Syndrome Protein and SCAR Homolog), is a nucleation-promoting factor at the surface of endosomes. It recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of endosomes. It associates with tubulin and localizes to early and recycling endosomes, where together with the Arp2/3 complex and actin, it is required for maintaining the shape of the endosomal compartment and the regulation of the retrograde transport.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Embryonic lethality in mice lacking Trim59 due to impaired gastrulation development
Su X, et al.
Cell Death & Disease, 9(3), 302-302 (2018)
WASH and the Arp2/3 complex regulate endosome shape and trafficking
Duleh SN and Welch MaD
Cytoskeleton (Hoboken, N.J.), 67(3), 193-206 (2010)
A FAM21-containing WASH complex regulates retromer-dependent sorting
Gomez TS and Billadeau DD
Developmental Cell, 17(5), 699-711 (2009)
WASH and WAVE actin regulators of the Wiskott-Aldrich syndrome protein (WASP) family are controlled by analogous structurally related complexes
Jia D, et al.
Proceedings of the National Academy of Sciences of the USA, 107(23), 10442-10447 (2010)
The hereditary spastic paraplegia protein strumpellin: characterisation in neurons and of the effect of disease mutations on WASH complex assembly and function
Freeman C, et al.
Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1832(1), 160-173 (2013)

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