SAB4200173

Anti-Rab18 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-RAB18LI1, Anti-ras-related protein Rab-18
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: ~23 kDa (an additional band at ∼43 kDa may appear in some extract preparations)
• species reactivity: rat, mouse, human
• concentration: ~1.0 mg/mL
• technique(s): immunoprecipitation (IP): 5-10 μg using lysates of human PC3 cells; indirect immunofluorescence: 2.5-5.0 μg/mL using rat NRK cells; western blot: 1-2 μg/mL using whole extracts of mouse MM3MG cells
• UniProt accession no.: Q9NP72
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... RAB18(22931) ; mouse ... Rab18(19330) ; rat ... Rab18(307039)

Description

General description

Rab18 is a member of the Rab family of small guanosine triphosphatases (GTPases). The Rab family belongs to the Ras superfamily of small guanosine triphosphatases (GTPases). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hypervariable C-terminal domains with a cysteine motif.

Application

Anti-Rab18 antibody produced in rabbit has been used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

Biochem/physiol Actions

Rab guanosine triphosphatases (Rab GTPases) are central regulators of membrane trafficking between different subcellular compartments of eukaryotic cells. Their regulatory capacity depends on their ability to cycle between the guanosine diphosphate (GDP)-bound inactive and GTP-bound active states. Activation of a Rab protein is coupled to its association with intracellular membranes. Through their effector proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement and membrane fusion. The conserved regions of Rab proteins are implicated in subcellular targeting. Each Rab protein shows a characteristic subcellular distribution. Therefore, antibodies to Rab proteins may serve as useful tool for studying subcellular localization and membrane organization. Rab18 is involved in retrograde Golgi-endoplasmic reticulum (ER) trafficking.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable