SAB4200124

Anti-Nexilin antibody,Mouse monoclonal

clone NX-38, purified from hybridoma cell culture

• Synonym(s): Anti-NEXN, Anti-Nelin
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: NX-38, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 80 kDa
• species reactivity: mouse, human, rat
• packaging: antibody small pack of 25 μL
• concentration: ~1 mg/mL
• technique(s): immunocytochemistry: suitable; western blot: 1-2 μg/mL using using rat brain S1 fraction extracts
• isotype: IgG2a
• UniProt accession no.: Q0ZGT2
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NEXN(91624) ; mouse ... Nexn(68810) ; rat ... Nexn(246172)

Description

General description

Monoclonal Anti-Nexilin (mouse IgG2a isotype) is derived from the hybridoma NX-38 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide conjugated to KLH. Nexilin is a filamentous actin-binding protein that localizes to focal contacts. Alternative splicing of the gene yields two different isoforms namely, b-nexilin and s-nexilin.

NEXN (nexilin), a cardiac Z-disc protein is made of 675 amino acids. It is present in the heart and skeletal muscle. NEXN is mapped to human chromosome 1p31.

Specificity

Monoclonal Anti-Nexilin recognizes human, rat, and mouse nexilin.

Application

Anti-Nexilin antibody,Mouse monoclonal may be used in immunoblotting and immunocytochemistry.

Biochem/physiol Actions

NEXN (nexilin) plays an important role in protecting cardiac Z-discs from forces generated within the sarcomere. Mutations in NEXN results in hypertrophic cardiomyopathy.

Nexilin may be involved in cell adhesion and migration.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable