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Key Documents

S400HR

Sigma-Aldrich

Sephacryl® Size Exclusion Resin

400-HR, MW range 20-8000 kDa (globular proteins)

Synonym(s):

Poly([allyl dextran]-co-N,N′-methylenebisacrylamide)

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About This Item

CAS Number:
65546-95-4
MDL number:
MFCD00165824
UNSPSC Code:
23151817
NACRES:
NA.56

product name

Sephacryl®, 400-HR, MW range 20-8000 kDa (globular proteins)

description

stability: stable to 0.2M NaOH

form

suspension (20% aqueous ethanol)

feature

autoclavable

matrix

crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer

particle size

25-75 μm (wet)

pore size

10-2000 kDa MW range (dextrans)
20-8000 kDa MW range (globular proteins)

compatibility

mode of use size exclusion chromatography

storage temp.

2-8°C

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General description

S400HR-750ML′s updated product is GE17-0609-01
  • Supplied swollen, suspended in 20% aqueous ethanol
  • Autoclavable
  • Stable to 0.2N sodium hydroxide (NaOH)
  • Store at refrigerated temperature (2-8 °C)

Application

Sephacryl® is used in gel filtration chromatography, protein chromatography, and resins and separation media. Sephacryl® has been used to develop a novel high-throughput screening assay for sickle cell disease drug discovery. Sephacryl® has also been used to purify and characterize enzymes such as bovine pancreatic elastase, tissue-nonspecific alkaline phosphatases and a lysozyme from human urine.
For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans..

Legal Information

Sephacryl is a registered trademark of Cytiva

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Hazard Classifications

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Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

flash_point_f

100.4 - 109.4 °F - closed cup

flash_point_c

38 - 43 °C - closed cup

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Enhancement of drug delivery to bone: Characterization of human tissue-nonspecific alkaline phosphatase tagged with an acidic oligopeptide
Nishioka T, et al.
Molecular Genetics and Metabolism, 88(3), 244-255 (2006)
Michael J Lodes et al.
Methods in molecular medicine, 94, 91-106 (2004-02-13)
Expression cloning involves the selection of specific polypeptides, generated from a cDNA or genomic DNA library, based on certain characteristics of the expressed proteins, such as antibody or ligand binding, recognition by T-cells, function, or complementation of cell defects. Here
E Käslan et al.
The Journal of biological chemistry, 269(19), 14103-14110 (1994-05-13)
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Journal of virology, 81(17), 8967-8976 (2007-06-15)
The Ebola virus (EBOV) genome encodes for several proteins that are necessary and sufficient for replication and transcription of the viral RNAs in vitro; NP, VP30, VP35, and L. VP30 acts in trans with an RNA secondary structure upstream of
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