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S0638

Sigma-Aldrich

Serum Replacement 1 (50×)

liquid, sterile-filtered, suitable for cell culture

Synonym(s):

50× Serum Replacement, Cell Culture Serum Substitute, Replacement Serum

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

Application

Serum Replacement 1 (50×) has been used as a supplement in Ham′s/F-12 medium for tumour cells. It has also been used as a supplement to culture Jurkat T cells in RPMI medium.(2)

Other Notes

Defined, multi-purpose serum replacement useful in studying the effects of growth factors and the production of proteins. Contains highly purified, heat-treated bovine serum albumin, heat-treated bovine transferrin and bovine insulin. Does not contain growth factors, steroid hormones, glucocorticoids, cell adhesion factors, detectable Ig and mitogens. May be used with anchorage-dependent and suspension cells.

Caution

Stable for 30 days when diluted in cell culture medium.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


Certificates of Analysis (COA)

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Proliferation and intracellular glutathione in Jurkat T cells with concentrated whey protein products
Middleton N, et al.
International Journal of Dairy Technology, 13(7), 565-573 (2003)
Constanze M Hammerle et al.
PLoS genetics, 16(10), e1009069-e1009069 (2020-10-16)
The genetic mechanisms that determine the size of the adult pancreas are poorly understood. Imprinted genes, which are expressed in a parent-of-origin-specific manner, are known to have important roles in development, growth and metabolism. However, our knowledge regarding their roles

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