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R4018

Sigma-Aldrich

Ribonucleic acid, transfer, phenylalanine specific from brewer′s yeast

Synonym(s):

Transfer RNA, Yeast Phe tRNA, tRNA

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About This Item

MDL number:
MFCD00132200
UNSPSC Code:
12352200
NACRES:
NA.55

grade

for molecular biology

form

lyophilized powder

mol wt

25  kDa

solubility

water: 2 mg/mL

storage temp.

2-8°C

General description

Transfer RNA is isolated from brewer′s yeast. This transfer RNA is specific for transfer of the amino acid phenylalanine.

The phenylalanine acceptor activity of 100% pure tRNA is 1,800 pmoles per A260 unit. Comparison of this number to lot-specific analysis may be used as an indication of product purity. Methods to further purify tRNA have been published.

The assay for this product is based on a published method. The activity is measured using a crude preparation of phenylalanyl transfer ribonucleic acid synthetase. The phenylalanine specific tRNA is used as a substrate for the enzyme in the assay.

This product is not suitable for use as carrier tRNA to aid in the ethanol precipitation of very small amounts of DNA. Product Nos. R 8508 and R 5636 are recommended for use as carrier tRNA for DNA purification and precipitation.

Application

Suitable for use:
  • As a tRNA standard
  • As a substrate in tRNA synthatase assays
  • As a substrate in other reactions involving tRNAs

Components

tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.

Preparation Note

Prepared by method of Wimmer, E., et al., Biochemistry, 7, 2623 (1968).

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mariana Pavon-Eternod et al.
Nucleic acids research, 37(21), 7268-7280 (2009-09-29)
Increased proliferation and elevated levels of protein synthesis are characteristics of transformed and tumor cells. Though components of the translation machinery are often misregulated in cancers, what role tRNA plays in cancer cells has not been explored. We compare genome-wide
W Yan et al.
The EMBO journal, 17(16), 4809-4817 (1998-08-26)
Correct folding of newly synthesized polypeptides is thought to be facilitated by Hsp70 molecular chaperones in conjunction with DnaJ cohort proteins. In Saccharomyces cerevisiae, SSB proteins are ribosome-associated Hsp70s which interact with the newly synthesized nascent polypeptide chain. Here we
Tissue-specific differences in human transfer RNA expression.
Dittmar, K.A., et al.
PLoS Genetics, 2, 2107-2107 (2006)
Abstracts of Albany 2013: the 18th Conversation. June 11-18, 2013. Albany, New York, USA.
Journal of biomolecular structure & dynamics, 31 Suppl 1, 1-139 (2013-06-06)
Hilal Kazan et al.
Nucleic acids research, 41(Web Server issue), W180-W186 (2013-06-12)
RBPmotif web server (http://www.rnamotif.org) implements tools to identify binding preferences of RNA-binding proteins (RBPs). Given a set of sequences that are known to be bound and unbound by the RBP of interest, RBPmotif provides two types of analysis: (i) de
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