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biological source
rat dermis
Quality Level
packaging
pkg of 500,000 cells
manufacturer/tradename
Cell Applications, Inc
growth mode
Adherent
morphology
Fibroblast
technique(s)
cell culture | mammalian: suitable
storage temp.
−196°C
General description
Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.
RDF from Cell Applications, Inc. have been used in allogenic prosthetic grafts, demonstrating improved epithelization, collagen organization and faster regeneration (Nomoto, 2012), in a cell sheet engineering study, where a sheet of dermal fibroblasts was transplanted into the damaged liver in allogeneic rat model to promote migration of hepatic progenitor cells and faster healing (Muraoka, 2013), and as a control in engineered cardiac tissues (Serrao, 2012).
RDF from Cell Applications, Inc. have been used in allogenic prosthetic grafts, demonstrating improved epithelization, collagen organization and faster regeneration (Nomoto, 2012), in a cell sheet engineering study, where a sheet of dermal fibroblasts was transplanted into the damaged liver in allogeneic rat model to promote migration of hepatic progenitor cells and faster healing (Muraoka, 2013), and as a control in engineered cardiac tissues (Serrao, 2012).
Cell Line Origin
Skin
Application
Injury recovery, connective tissue research, laminin and fibronectin production, extracellular matrix, tissue organization, wound healing, fibroblasts growth factor response.
Components
Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO
Quality
Each lot was tested for proper morphology, Population Doublings, Negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.
Preparation Note
- 1st passage, >500,000 cells in Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO
- Can be cultured at least 8 population doublings
Subculture Routine
Please refer to the RDF Culture Protocol.
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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