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R0406

Sigma-Aldrich

Anti-RUNX1 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Acute myeloid leukemia 1 protein, AML-1, Anti-CBF-α2, Anti-Core-binding Factor α2 subunit, Anti-Oncogne AML-1, Anti-PEBP2-αB, PEA2-αB, Anti-Polyomavirus enhancer-binding protein 2αB subunit, Anti-Runt-related transcription factor 1, Anti-SL3-3 enhancer factor 2αB subunit, Anti-SL3/AKV core-binding factor αB subunit

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About This Item

MDL number:
MFCD07370485
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~48 kDa

species reactivity

human, mouse, rat (predicted)

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde-fixed HeLa cells
western blot: 0.25-0.5 μg/mL using HeLa cell lysate

UniProt accession no.

Q01196

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RUNX1(861)
mouse ... Runx1(12394)
rat ... Runx1(50662)

General description

RUNX1 encodes a DNA-binding subunit.
Runt-related transcription factor 1 (RUNX1) is a transcription factor that plays an important role in hematopoiesis, osteogenesis and neurogenesis. It is a member of Runt-related transcription factors (RUNXs). It was first identified as a component of Polyomavirus enhancer binding protein 2 (PEBP2) and Moloney murine leukemia virus enhancer core binding factor (CBF). Three isoforms of the protein have been identified: RUNX1a, RUNX1b and RUNX1c. Structurally, it consists of Runt domain at the N-terminal region.

Immunogen

synthetic peptide corresponding to amino acids 42-52 of human RUNX1, conjugated to KLH. The corresponding sequence is identical in rat and mouse.

Application

Anti-RUNX1 (N-terminal) antibody produced in rabbit is suitable for indirect immunofluorescence and western blot.

Biochem/physiol Actions

RUNX activity is regulated by extracellular signaling pathways resulting in post-translational modifications, such as phosphorylation, acetylation and ubiquitination. It is linked to acute leukemia.
RUNX1 (Runt-related transcription factor 1) plays a vital role in tumour suppression and oncogenic activities. During hematopoiesis, it is involved in hematopoietic development, hematopoietic stem cell homeostasis, and various blood malignancies. It may have clinicopathological impact on the proliferation of human bone marrow cells used for transplantation therapy.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Phosphorylation, acetylation and ubiquitination: the molecular basis of RUNX regulation
Bae SC and Lee YH
Gene, 366(1), 58-66 (2006)
Kentson Lam et al.
Frontiers in bioscience (Landmark edition), 17, 1120-1139 (2011-12-29)
RUNX1 is a transcription factor that regulates critical processes in many aspects of hematopoiesis. RUNX1 is also integral in defining the definitive hematopoietic stem cell. In addition, many hematological diseases like myelodysplastic syndrome and myeloproliferative neoplasms have been associated with
Ming Zhuang et al.
Biochemical and biophysical research communications, 448(3), 315-322 (2014-01-07)
The lncRNA H19 has been recently shown to be upregulated and play important roles in gastric cancer tumorigenesis. However, the precise molecular mechanism of H19 and its mature product miR-675 in the carcinogenesis of gastric cancer remains unclear. In this
Hiroshi Yajima et al.
BMC biology, 12, 40-40 (2014-06-03)
Various senses and sensory nerve architectures of animals have evolved during adaptation to exploit diverse environments. In craniates, the trunk sensory system has evolved from simple mechanosensory neurons inside the spinal cord (intramedullary), called Rohon-Beard (RB) cells, to multimodal sensory
Yu Fei Lee et al.
PloS one, 9(9), e106661-e106661 (2014-09-05)
The orchestration of histone modifiers is required to establish the epigenomic status that regulates gene expression during development. Whsc1 (Wolf-Hirschhorn Syndrome candidate 1), a histone H3 lysine 36 (H3K36) trimethyltransferase, is one of the major genes associated with Wolf-Hirshhorn syndrome

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