P7373

PAK7 Active human

≥75% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

• Synonym(s): PAK5, p21 activated kinase-7
• MDL number: MFCD07371026
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• assay: ≥75% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 10 units/mg protein (Bradford)
• mol wt: protein apparent mol wt ~116 kDa by SDS-PAGE
• UniProt accession no.: Q9P286
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... PAK7(57144)

Description

Biochem/physiol Actions

PAK7 is a PAK family kinase that contains a CDC42/Rac1 interactive binding (CRIB) motif at the N-terminus and a Ste20-like kinase domain at the C-terminus. PAK7 has been implicated in the regulation of cell morphology, motility and transformation. PAK7 is moderately expressed in adult and fetal whole brain and in all specific adult brain regions tested.

Unit Definition

One unit will incorporate one nanomole of phosphate into the peptide substrate CKRPRAASFAE per minute at 30 °C and pH 7.2 using a final concentration of 50 μM [32P] ATP.

Physical form

Solution in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, and 30% glycerol.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)