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Key Documents

P3338

Sigma-Aldrich

Prostate Specific Antigen from human semen

buffered aqueous solution

Synonym(s):

Cancer-associated PSA, PSA

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About This Item

MDL number:
UNSPSC Code:
12352202
NACRES:
NA.32

Quality Level

UniProt accession no.

storage temp.

2-8°C

Gene Information

human ... KLK3(354)

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General description

Prostate specific antigen (PSA) is a 28.43 kDa single-chain glycoprotein containing 237 amino acids, encoded by the kallikrein gene mapped to human chromosome 19. It is specifically expressed by human prostate tissue and is one of the highly abundant proteolytic enzyme expressed in the seminal fluid.

Application

Prostate Specific Antigen from human semen has been used:
  • in microarray sandwich assay
  • in the preparation of sandwich immunosensor
  • as a target protein to test composite organic−inorganic nanoparticle (COIN) and Alexa conjugates in a plate-binding assay

Biochem/physiol Actions

Prostate Specific Antigen (PSA), a glycoprotein of the glandular kallikrein family, is a serine protease with chymotrypsin-like enzymatic activity. Circulating PSA exists in free and protease inhibitor-bound forms. Studying the ratios of free to inhibitor-bound forms may prove valuable in the diagnosis of prostate cancer.
Prostate-specific antigen (PSA) is an androgen-regulated serine protease specifically produced by prostatic tissue and is secreted into the lumen. PSA in semen cleaves semenogelins present in the seminal coagulum. Total PSA levels are increased in serum of prostate cancer (PCa) patients. PSA functions as a potential biomarker for PCa. Serum protease inhibitors such as α1-antichymotrypsin (α1-ACT), α2-macroglobulin (α2-M), and other acute-phase proteins form irreversible complexes with circulating PSA and hinder proteolytic activity of PSA.

Packaging

Package size based on protein content

Quality

Highly purified

Physical form

Solution in phosphate buffered saline, contains 0.05-0.1% sodium azide with 50% glycerol

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Microarray-based multiplexed scanometric immunoassay for protein cancer markers using gold nanoparticle probes.
Kim D
Analytical Chemistry, 81(21), 183-187 (2009)
Barry Lutz et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 56(4), 371-379 (2007-12-12)
Surface-enhanced Raman scattering (SERS) nanoparticles are emerging as a new approach for optical detection of biomolecules. In a model assay in formalin-fixed paraffin-embedded (FFPE) prostate tissue sections, we detect prostate-specific antigen (PSA) using antibody (Ab) conjugated to composite organic-inorganic nanoparticles
Raman nanoparticle probes for antibody-based protein detection in tissues
Lutz B, et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 56(4), 371-379 (2008)
Usefulness of total PSA value in prostate diseases diagnosis
Prcic A, et al.
Acta Informatica Medica, 24(3), 156-156 (2016)
A M Zhou et al.
Clinical chemistry, 39(12), 2483-2491 (1993-12-01)
Prostate-specific antigen (PSA) in serum is primarily complexed with alpha 1-antichymotrypsin (alpha 1-ACT). However, 12-15% of prostate cancer (PCa) patients present with the predominant form being uncomplexed (free) PSA (Lilja et al., Clin Chem 1991;37:1618-24). We report that commercial immunoassays

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