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Key Documents

MBD0026

Sigma-Aldrich

Microbial community DNA mix

Suitable for PCR, sequencing and NGS, 10 ng/μL

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About This Item

UNSPSC Code:
41105500
NACRES:
NA.51

Quality Level

200

form

liquid

concentration

10 ng/μL

technique(s)

DNA extraction: suitable
DNA sequencing: suitable
PCR: suitable

shipped in

dry ice

storage temp.

−20°C

General description

Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses1.
The Microbial community DNA mix is composed of defined collection of genomic DNA standards from 10 different bacterial species, with ranging genome sizes and GC content and varying phyla. This collection can serve as standard for benchmarking the performance along the workflow of microbiomics and as a tool to increase reproducibility and allow comparison of results obtained by different labs.


Read here how to use our standards to ensure data integrity for your microbiome research.

Application

The genomic DNA is provided at ≥10 ng/μl concentration in TE buffer pH 8.0.It is recommended to avoid freeze thaw cycles of this product.

Features and Benefits

  • Microbial mix for microbiomics and meta- genomics workflow
  • Suitable standard for PCR, sequencing and NGS
  • Improve Bioinformatics analyses
  • Increases reproducibility
  • Compare results lab to lab

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Articles

DNA Standards for Metagenomic Research into Microbiome Communities

The use of standards is critical to the integrity of metagenomics research. Learn how DNA standards for bacteria, fungi, and viruses are applied to studying the microbiome. Choose standards for E. coli and other key species, as well as mixed community standards.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service