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Key Documents

MAK437

Sigma-Aldrich

Glutathione Peroxidase Assay Kit

Sufficient for 100 colorimetric tests

Synonym(s):

GPx Activity Assay Kit, GPx Activity Detection Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

Quality Level

detection method

colorimetric

relevant disease(s)

cancer, diabetes; endocrinological disorders

storage temp.

−20°C

General description

Glutathione peroxidase (GPX) represents an enzyme family with peroxidase activity whose main biological role is to protect the organism from oxidative damage. It helps prevent lipid peroxidation of cellular membranes by removing free peroxide in the cell.

Application

The Glutathione Peroxidase Assay Kit may be used for:
  • Cancer Research
  • Diabetes Research
  • Oxidative Stress Research

Features and Benefits

Enhanced Detection Range: Detect GPx activity accurately across a wide range, from 40 to 800 U/L, using only 10 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirement.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

Suitability

The kit is suitable for the quantitative determination of glutathione peroxidase activity and evaluation of drug effects on GPX activity in biological samples.

Principle

The Glutathione Peroxidase Assay Kit directly measures NADPH consumption in the enzyme coupled reactions. The measured decrease in optical density at 340 nm (A340) is directly proportional to the enzyme activity in the sample.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

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Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Org. Perox. F - Skin Corr. 1B - STOT RE 2 Inhalation - STOT SE 3

target_organs

Lungs, Respiratory system

Storage Class

5.2 - Organic peroxides and self-reacting hazardous materials

flash_point_f

174.2 °F

flash_point_c

79 °C


Certificates of Analysis (COA)

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