MAK321

Acetaldehyde Assay Kit

sufficient for 100 fluorometric tests

• Synonym(s): Acetaldehyde Quantitation Kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• usage: sufficient for 100 fluorometric tests
• input: culture(s); food(s); serum; plasma; tissue; urine; beverage(s)
• application(s): cosmetics; food and beverages
• detection method: fluorometric
• relevant disease(s): gastrointestinal diseases
• storage temp.: −20°C

Description

General description

Acetaldehyde is one of the most widely occurring aldehydes in nature and commonly used in industry. The metabolic byproduct of ethanol in the liver, acetaldehyde is toxic to the human body and rapidly converted to the less harmful acetic acid by the enzyme aldehyde dehydrogenase. People with a deficiency of aldehyde dehydrogenase accumulate acetaldehyde when consuming alcohol and this accumulation results in facial and body flushing known as alcohol flush reaction. Build up of acetaldehyde has also been associated with the effects of hangovers from alcohol consumption. Although classified as a carcinogen, acetaldehyde is naturally found in many foods and beverages such as ripe fruit, coffee, and wine.

Application

The Acetaldehyde Assay Kit may be used for:

• Food and Beverage Testing
• Gastrointestinal Disease Research

Features and Benefits

Enhanced Detection Range: Detect Acetaldehyde levels accurately across a wide range, from 0.5 µM to 60 µM, using only 50 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirement.

Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a 30 minute room temperature reaction, reducing complexity and saving valuable time and effort.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

Suitability

Suitable for biological samples (e.g. plasma, serum, urine, tissue and culture media) and food/beverage samples (e.g. wine, coffee, and juice)

Principle

The fluorimetric acetaldehyde assay is based on aldehyde dehydrogenase catalyzed oxidation of acetaldehyde, in which the generated NADH reduces a probe making it fluorescent. The fluorescence intensity of the product measured at λex/em = 530/585 nm is directly proportional to acetaldehyde concentration in the sample.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Safety Information

• pictograms: GHS05
• signalword: Danger
• hcodes: H290,H314
• pcodes: P234 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
• Hazard Classifications: Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B
• Storage Class: 8A - Combustible corrosive hazardous materials