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MAK112

Sigma-Aldrich

Arginase Activity Assay Kit

sufficient for 200 colorimetric tests

Synonym(s):

Arginase Test Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 200 colorimetric tests

detection method

colorimetric

relevant disease(s)

cancer; immunological diseases; gastrointestinal diseases

storage temp.

−20°C

Gene Information

human ... ARG1(383) , ARG2(384)
mouse ... ARG1(11846) , ARG2(11847)
rat ... ARG2(29215) , Arg1(29221)

General description

Arginase is a manganese-containing enzyme that catalyzes the conversion of arginine to urea and ornithine. Two isoforms of arginase are present in most mammals which differ in their tissue distribution and subcellular localization. Arginase I is a cytoplasmic protein predominantly expressed in the liver where it catalyzes the fifth and final step of the Urea Cycle. Arginase II is a mitochondrial protein with broader tissue distribution whose function may include nitric oxide and polyamine metabolism. Decreased activity or expression of Arginase I results in the autosomal recessive disorder hyperarginemia. Increased serum Arginase activity is implicated in hepatic injury and in certain pathological conditions such as cancer. Arginase also participates in multiple inflammatory reactions in the immune system, such as macrophage-mediated cytotoxicity.

Application

Arginase Activity Assay Kit has been used to assess the activity of arginase.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the detection of arginase activity in enzyme preparations, serum, plasma, tissue culture, and similar samples. Suitable for studying the effects of drugs on arginase activity.

Principle

In this assay, arginase catalyzes the conversion of arginine to urea and ornithine. The urea produced specifically reacts with the substrate to generate a colored product, proportional to the arginase activity present.

pictograms

Health hazardCorrosion
GHS08,GHS05

signalword

Danger

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Repr. 1B - Skin Corr. 1A

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Arginase activity, urea, and hydroxyproline concentration are reduced in keratoconus keratocytes.
Stachon T, et al.
Graefe'S Archive For Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Fur Klinische Und Experimentelle Ophthalmologie, 255(1), 91-97 (2017)
Clinical phenotype, biochemical profile, and treatment in 19 patients with arginase 1 deficiency.
Martina H.
Journal of inherited metabolic disease, 39(3), 331?340-331?340 (2016)
Melanoma exosomes promote mixed M1 and M2 macrophage polarization.
Bardi G T, et al.
Cytokine, 105, 63-72 (2018)
Max Hübner et al.
Critical care medicine, 47(8), e700-e709 (2019-06-01)
Cardiopulmonary bypass is associated with severe immune dysfunctions. Particularly, a cardiopulmonary bypass-related long-lasting immunosuppressive state predisposes patients to a higher risk of postoperative complications, such as persistent bacterial infections. This study was conducted to elucidate mechanisms of post-cardiopulmonary bypass immunosuppression.
Sarah Pasquin et al.
Journal of immunology (Baltimore, Md. : 1950), 201(8), 2462-2471 (2018-09-14)
CLCF1 is a neurotrophic and B cell-stimulating factor belonging to the IL-6 family. Mutations in the gene coding for CLCF1 or its secretion partner CRLF1 lead to the development of severe phenotypes, suggesting important nonredundant roles in development, metabolism, and
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