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MAK107

Sigma-Aldrich

LCAT Activity Assay Kit

Supplied by Roar Biomedical, Inc.

Synonym(s):

Lecithin:cholesterol acyltransferase activity assay kit

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About This Item

EC Number:
200-659-6
UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 fluorometric tests

application(s)

pharmaceutical

detection method

fluorometric

relevant disease(s)

cardiovascular diseases

storage temp.

−20°C

Gene Information

human ... LCAT(3931)

General description

The plasma protein lecithin:cholesterol acyltransferase (LCAT) catalyzes the transfer of an acyl group from the sn2 position of phosphatidylcholine to the 3-hydroxyl group of cholesterol resulting in the formation cholesteryl ester. This enzymatic activity occurs on the surface of high density lipoprotein (HDL). The cholesteryl esters formed by LCAT may be packed into the core of HDL.

Application

Applications for this method include high-throughput screening, mechanism of action studies and structure-activity relationship (SAR) work.
LCAT Activity Assay Kit has been used for measuring lecithin-cholesterol acyl transferase activity in plasma samples.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for measuring phospholipase activity of LCAT in plasma or serum.

Principle

The LCAT Activity Assay Kit is a fluorometric assay useful for measuring phospholipase activity of (λEx=390 nm/λEm=470 nm) LCAT. The assay may be validated by inhibition of LCAT with iodoacetate or another spectrally benign (not Ellman′s reagent) LCAT inhibitor.

Analysis Note

Learn more about Roar Assay Kits for Studies in Lipoprotein Metabolism.

Kit Components Only

Product No.
Description
  • Substrate Reagent .1 mL
  • READ Reagent 30 mL
  • LCAT Assay Buffer 20 mL

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Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

Storage Class

3 - Flammable liquids

flash_point_f

49.5 °F

flash_point_c

9.7 °C

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

LCAT Activity Assay Kit

The LCAT Activity Assay Kit is a fluorometric assay useful for measuring phospholipase activity of LCAT.

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