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Key Documents

M6688

Sigma-Aldrich

Mimetic Blue AP Ligand Affinity Adsorbent

suspension

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About This Item

MDL number:
UNSPSC Code:
23151817
NACRES:
NA.56

form

suspension

Quality Level

shipped in

wet ice

storage temp.

2-8°C

Application

Ligand affinity adsorbents are used in protein chromatography, affinity chromatography and dye resins.

pictograms

Flame

signalword

Warning

hcodes

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

flash_point_f

95.0 °F - closed cup

flash_point_c

35 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


Certificates of Analysis (COA)

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Fei Liu et al.
Journal of immunological methods, 274(1-2), 115-127 (2003-03-01)
A quadroma (hybrid-hybridoma) secreting bispecific antibodies with one paratope specific for M13 bacteriophage coat protein and another paratope specific for alkaline phosphatase (AP) was developed by electro-fusion of the two parental hybridomas and selected by a fluorescence activated cell sorter
D Xu et al.
Journal of chromatography. B, Biomedical sciences and applications, 706(2), 217-229 (1998-04-29)
We developed a simple purification method to purify alkaline phosphatase/anti-alkaline phosphatase IgG as immune complexes using mimetic affinity chromatography wherein the antibody was either a monospecific antibody, a bispecific antibody or a commercial polyclonal IgG conjugated with alkaline phosphatase (AP-IgG)
Jeannie Horak et al.
Analytical and bioanalytical chemistry, 400(8), 2349-2359 (2010-12-22)
The new affinity-type Mimetic Ligand™ B14 was coupled with a 1,2-diaminoethane spacer (2LP) and a [1,2,3]-triazole spacer (TRZ) to three different support media. In addition to the agarose-based PuraBead and the polymethacrylate-type Fractogel, three new polymeric support media were introduced
S J Burton
Methods in molecular biology (Clifton, N.J.), 11, 91-103 (1992-01-01)
Dye-ligand affinity chromatography is performed with an adsorbent consisting of a solid support matrix to which a dye has been cova-lently bonded. The protein mixture is passed through a packed bed of adsorbent that selectively binds proteins that are able
C R Lowe et al.
Trends in biotechnology, 10(12), 442-448 (1992-12-01)
Affinity chromatography has been extensively refined over the past few years to meet the more stringent criteria being placed on recombinant proteins as therapeutic products. New developments in the design of selective and stable ligands for affinity chromatography are establishing

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