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M6190

Sigma-Aldrich

Monoclonal Anti-MYOD1 antibody produced in mouse

clone 5.2F, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-Myogenic Differentiation Antigen 1

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About This Item

MDL number:
MFCD01323984
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5.2F, monoclonal

form

buffered aqueous solution

mol wt

antigen 34 kDa

species reactivity

human, rat, chicken, mouse

concentration

1.0 mg/mL

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2-4 μg/mL
immunohistochemistry (frozen sections): 2-4 μg/mL
immunoprecipitation (IP): 2 μg using 1 mg protein lysate
western blot: 1 μg/mL (reacts with the ~45 kDa protein)

isotype

IgG2a

UniProt accession no.

P15172

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... MYOD1(4654)
mouse ... Myod1(17927)
rat ... Myod1(337868)

General description

Myogenic differentiation antigen 1 (MYOD1) is a nuclear protein which is expressed in skeletal muscles. It is part of the basic helix-loop-helix (bHLH) family of transcription factors. The gene encoding it is localized on human chromosome 11.

Immunogen

recombinant mouse MyoD1 protein.

Application

Monoclonal Anti-MYOD1 antibody produced in mouse has been used in:
  • immunofluorescence staining at a 1:50 dilution
  • western blotting
  • immunostaining at a 1:300 dilution

Biochem/physiol Actions

Myogenic differentiation antigen 1 (MYOD1) maybe involved in recruitment of enzymes like acetyltransferases and methyltransferases to myogenic enhancers in the human genome. It takes part in the regeneration of muscles and mediates muscle cell differentiation by activating cell cycle arrest.

Physical form

Solution in phosphate buffered saline containing 0.08% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Roy Blum
Journal of cellular biochemistry, 115(11), 1855-1867 (2014-06-07)
The early 1980s revelation of cis-acting genomic elements, known as transcriptional enhancers, is still regarded as one of the fundamental discoveries in the genomic field. However, only with the emergence of genome-wide techniques has the genuine biological scope of enhancers
Ramesh P Arasaradnam et al.
BMC research notes, 5, 240-240 (2012-05-18)
DNA methylation of promoter-associated CpG islands of certain genes may play a role in the development of colorectal cancer. The MYOD-1 gene which is a muscle differentiation gene has been showed to be significantly methylated in colorectal cancer which, is
Jennifer A Tinklenberg et al.
Journal of neuropathology and experimental neurology, 78(2), 130-139 (2019-01-01)
Mutations in at least 12 genes are responsible for a group of congenital skeletal muscle diseases known as nemaline myopathies (NMs). NMs are associated with a range of clinical symptoms and pathological changes often including the presence of cytoplasmic rod-like
Bahar Shahidi et al.
JOR spine, 3(2), e1087-e1087 (2020-07-03)
Many chronic musculoskeletal conditions are associated with loss of muscle volume and quality, resulting in functional decline. While atrophy has long been implicated as the mechanism of muscle loss in these conditions, recent evidence has emerged demonstrating a degenerative phenotype
Narasimhan P Agaram et al.
Genes, chromosomes & cancer, 53(9), 779-787 (2014-05-16)
Sclerosing and spindle cell rhabdomyosarcoma (RMS) are rare types of RMS recently reclassified as a stand-alone pathologic entity, separate from embryonal RMS (ERMS). Although sclerosing and spindle cell RMS share clinical and morphologic features, a pathogenetic link based on shared
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