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Key Documents

L2785

Sigma-Aldrich

Lectin from Dolichos biflorus (horse gram)

lyophilized powder

Synonym(s):

Dolichos biflorus agglutinin, DBA

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

form

lyophilized powder

potency

≤20 μg per mL agglutination activity

impurities

salt, essentially free

storage temp.

−20°C

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Biochem/physiol Actions

DBA has anti-A1 human blood group specificity, useful for distinguishing between A1 and A2 blood types. DBA has strong anti-Cad activity, and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues. Conjugates are prepared from affinity purified lectin.

Preparation Note

Purified by affinity chromatography.

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A1 erythrocytes after 1 hour incubation at 25 °C.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ann Marie Hynes et al.
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