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I6505

Sigma-Aldrich

Diethylaminoethyl–Sephacel®

aqueous ethanol suspension, 40-160 μm (wet), exclusion limit ~1,000,000 Da

Synonym(s):

DEAE-Sephacel®

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About This Item

CAS Number:
9013-34-7
MDL number:
MFCD00146622
UNSPSC Code:
47101511
PubChem Substance ID:
24896090
NACRES:
NA.56

form

aqueous ethanol suspension

Quality Level

200

technique(s)

activity assay: suitable

particle size

40-160 μm (wet)

pore size

~1,000,000 Da exclusion limit

pH

2—12

capacity

100-140 μeq/mL, gel

storage temp.

2-8°C

SMILES string

OC[C@@H]1O[C@@H](O[C@H]2[C@@H](O)[C@H](O)[C@@H](O)O[C@H]2CO)[C@@H](O)[C@H](O)[C@H]1O

InChI

1S/C12H22O11/c13-1-3-5(15)6(16)9(19)12(22-3)23-10-4(2-14)21-11(20)8(18)7(10)17/h3-20H,1-2H2/t3?,4?,5?,6?,7?,8?,9?,10-,11?,12+/m1/s1

InChI key

GUBGYTABKSRVRQ-WFVLMXAXSA-N

General description

I6505-500ML′s update product number is GE17-0500-01

Application

Diethylaminoethyl (DEAE) Sephacel® is used in protein chromatography, ion exchange chromatography, and anion exchange media. It has been used to study sperm motility, sperm capacitation and sperm-oocyte interactions. DEAE Sephacel has also been used to study patients with immunoglobulin A (IgA) nephropathy. Diethylaminoethyl–Sephacel® has been used:

  • in the purification of media samples by ion-exchange chromatography
  • in the isolation of glycosaminoglycans (GAG) from cultured animal cells
  • in the purification of cytotoxic fractions from Tritrichomonas foetus isolates

Physical form

Suspension in 20% ethanol

Legal Information

Sephacel is a registered trademark of Cytiva

Related product

Product No.
Description
Pricing

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

flash_point_f

129.2 °F

flash_point_c

54 °C

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The spectroscopic and photochemical properties of locked-15,15'-cis-spheroidene in solution and incorporated into the reaction center of <I>Rhodobacter sphaeroides</I> R-26.1.
Bautista, J.A., et al.
Photosynthesis Research, 55(1), 49-65 (1998)
Yuan-Hong Wang et al.
Acta biochimica et biophysica Sinica, 38(9), 633-638 (2006-09-06)
Extracellular alginate lyase secreted by marine Vibrio sp. YWA, isolated from decayed Laminaria japonica, was purified by a combination of ammonium sulfate precipitation and diethylaminoethyl-Sephacel column chromatography. The results show that the molecular mass of alginate lyase was approximately 62.5
V T D'Souza et al.
Biochemical and biophysical research communications, 129(3), 727-732 (1985-06-28)
An artificial chymotrypsin, with all the features of the real chymotrypsin, namely a binding site (from cyclodextrin) attached to a catalytic site containing an imidazolyl group, a carboxylate group and a hydroxyl group, has been synthesized. This artificial chymotrypsin has
A Ortiz et al.
Nucleic acids research, 24(16), 3280-3281 (1996-08-15)
A simple method is presented to circumvent laborious and time consuming electrophoretic separations of specific PCR amplification products. Specific target DNA is amplified using nucleotides labelled with DIG-dUTP or biotin-dCTP. The labelled PCR products are separated from unincorporated nucleotides or
H A Frank et al.
Photosynthesis research, 37(3), 193-203 (1993-09-01)
Triplet state electron paramagnetic resonance (EPR) experiments have been carried out at X-band on Rb. sphaeroides R-26 reaction centers that have been reconstituted with the carotenoid, spheroidene, and exchanged with 13(2)-OH-Zn-bacteriochlorophyll a and [3-vinyl]-13(2)-OH-bacteriochlorophyll a at the monomeric, 'accessory' bacteriochlorophyll
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