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H7786

Sigma-Aldrich

Hck human

recombinant, expressed in baculovirus infected insect cells, buffered aqueous glycerol solution

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About This Item

MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in baculovirus infected insect cells

Quality Level

form

buffered aqueous glycerol solution

specific activity

≥350 units/mg protein

mol wt

58 kDa

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... HCK(3055)

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Biochem/physiol Actions

Hck is a member of the Src family of non-receptor associated protein tyrosine kinases (PTK).

Unit Definition

One unit will transfer 1 nanomole of phosphate per minute using polyE4Y as the substrate at pH 7.5 at 30 °C

Physical form

Solution in 50 mM Tris pH 7.5, containing 0.05 mM EDTA, 1mM DTT, 100 mM NaCl, 0.05% NP-40, and 50% glycerol.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Catherine Astarie-Dequeker et al.
Journal of cell science, 115(Pt 1), 81-89 (2002-01-22)
In macrophages, lysosomes are suspected to have a heterogenous population of vesicles. This study was thus undertaken to identify and to characterize lysosomal compartments in human macrophages. Hck is a Src-family tyrosine kinase associated with secretory lysosomes in neutrophils and

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