Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

GERPN2125

ECL Blocking Agent

Cytiva RPN2125

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41105332
NACRES:
NA.32

feature

wetted part: no

packaging

pkg of 40 g

manufacturer/tradename

Cytiva RPN2125

color

White

storage temp.

2-8°C

General description

During the Western blotting process, non-specific binding of antibodies to the membrane is detrimental to the specificity and sensitivity of the assay. To overcome this challenge, it is essential to block parts of the membrane not already occupied by proteins. The Amersham ECL blocking agent can be used for blocking Amersham Protran nitrocellulose (NC) and Amersham Hybond polyvinylidene fluoride (PVDF) membranes when used along with the ECL chemiluminescence detection reagents, such as Amersham ECL, ECL start, ECL Prime, and ECL Select.

Application

This blocking agent is intended for blocking nitrocellulose and PVDF membranes used with ECL and ECL Plus Western Blotting Detection Reagents.

Features and Benefits

  • Each pack contains 40 g of membrane blocking agent.
  • Sufficient for at least 20 miniblots.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

ECL is a trademark of Cytiva

Storage Class

13 - Non Combustible Solids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Identification of Melanoma Antigens Using a Serological Proteome Approach (SERPA)
Ayako Suzuki, et al.
Cancer, Genomics, and Proteomics (2010)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service