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GE28-9574-99

TALON® Superflow™

Cytiva 28-9574-99, pack of 10 mL

Synonym(s):

TALON Affinity Resin

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About This Item

UNSPSC Code:
23151817
NACRES:
NA.56

ligand

TALON® Superflow Cobalt-IMAC

packaging

pack of 10 mL

manufacturer/tradename

Cytiva 28-9574-99

matrix

6% cross-linked agarose

particle size

60-160 μm

cleaning in place

2-14(Co2+-stripped medium)

working range

3-12(Co2+-stripped medium)

capacity

≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)

storage temp.

2-8°C

General description

Talon® Superflow is a cobalt-based IMAC medium offering different selectivity compared to nickel-charged media.

Features and Benefits

  • Cobalt-based chromatography medium designed for purification of histidine-tagged recombinant Proteins by immobilized metal affinity chromatography (IMAC).
  • Suitable for IMAC purification of histidine-tagged Proteins when Ni2+ is not the optimal choice of metal ion.
  • Compatible with commonly used IMAC reagents and appropriate for purifying Proteins under native or denaturing conditions.
  • Allows for Protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Superflow is a trademark of Sterogene Bioseparations Inc.
Talon is a registered trademark of Clontech

pictograms

Flame
GHS02

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Warning

hcodes

H226

Storage Class

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Manual Purification of Histidine-Tagged Proteins using His GraviTrap™ TALON® Columns

His GraviTrap™ TALON® columns are prepacked with 1 ml of TALON® Superflow medium. Each column provides simple manual purification of up to 15 mg of histidine-tagged proteins.

Performing a Cell Lysis of Recombinant Histidine-Tagged Proteins

For optimal conditions for growth, induction, and cell lysis of recombinant histidine-tagged proteins, please refer to established procedures. The following is a general procedure for cell lysis and sample preparation from bacterial cultures. Other established procedures may also work.

Optimizing Purification of Histidine-Tagged Proteins

How to optimize purification of histidine-tagged proteins using Cytiva products.

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

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Protocols

Purification of Histidine-Tagged Proteins using TALON® Superflow

This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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