GE17-5481-15

HiScreen^™ Capto^™ Core 700

Cytiva 17-5481-15, pkg of 1 column

• Synonym(s): Capto Core 700 Column
• UNSPSC Code: 23151817
• NACRES: NA.56

Properties

• ligand: octylamine
• packaging: pkg of 1 column
• manufacturer/tradename: Cytiva 17-5481-15
• parameter: 0.8-3.9 ml/min flow rate; 8 bar
• bed size: 77 mm × 100 mm
• bed volume: 4.7 mL
• column I.D.: 7.7 mm
• matrix: highly cross-linked agarose
• avg. part. size: 75 (d₅₀v is the median particle size of the cumulative volume distribution)
• cleaning: 3-13(pH interval where the medium can be subjected to, for cleaning- or sanitization-in-place.)
• working range: 2-14(pH interval where the medium can be operated without significant change in function.)
• capacity: ~13 mg binding capacity (ovalbumin)
• suitability: suitable for bioprocess medium

Description

General description

Capto Core 700 is designed for intermediate purification and polishing of viruses and other large biomolecules. The novel core bead technology and multimodal, octylamine ligand give Capto Core 700 dual functionality, size exclusion, and binding chromatography in one chromatography resin.

Capto Core 700 is composed of a ligand-activated core and inactive shell. The inactive shell excludes large molecules (cut off ~ Mr 700 000 [700 kDa]) from entering the core through the pores of the shell. These larger molecules are collected in the column flowthrough while smaller impurities bind to the internalized ligands. The core of each bead is functionalized with ligands that are both hydrophobic and positively charged, resulting in a highly efficient multimodal binding of various contaminants small enough to enter the core. The multimodal ligands ensure strong binding with most impurities over a wide range of pH and salt concentrations. Bound impurities are removed from the beads by cleaning-in-place (CIP) procedures using NaOH and in most cases, a solvent.

Dynamic binding capacities were measured at 10% breakthrough with a residence time of 3 min (1.6 ml/min = 200 cm/h) in HiScreen columns. Buffer: 20 mM Tris-HCl, 150 mM NaCl, pH 7.5.

Features and Benefits

• For intermediate purification and polishing of viruses and other large biomolecules (Mr > 700 000) in flowthrough mode.
• Novel core bead technology allows efficient capture of contaminants while target molecules are collected in the flowthrough.
• Significantly improved productivity and higher flow rates compared with gel filtration methods.
• Straightforward optimization due to flowthrough chromatography and robust performance.
• Convenient small-scale purification, process development, and scale-up using prepacked HiTrap and HiScreen columns.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Capto is a trademark of Cytiva

HiScreen is a trademark of Cytiva

Safety Information

• pictograms: GHS02
• signalword: Warning
• hcodes: H226
• pcodes: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class: 3 - Flammable liquids