GE17-5123-10
benzamidin Seph 4Fast Flow H-sub
Cytiva 17-5123-10, pack of 25 mL
About This Item
ligand
p-aminobenzamidine
packaging
pack of 25 mL
manufacturer/tradename
Cytiva 17-5123-10
storage condition
(0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol)
matrix
highly cross-linked 4% agarose
average diameter
90 μm
cleaning in place
1-9
working range
2-8
storage temp.
2-8°C
Related Categories
General description
Application
Features and Benefits
- Benzamidine Sepharose™ 4 Fast FLow (high sub) is based on highly cross-linked 4% Agarose enabling high fLow rates without losing binding capacity
- p-aminobenzamidine (pABA) immobilized on Sepharose™ 4 Fast FLow matrix gives high productivity, and allows easy scale-up.
- Effective removal of thrombin and factor Xa after tag cleavage of recombinant Proteins
- For convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.
Storage and Stability
Analysis Note
Legal Information
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
Certificates of Analysis (COA)
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Articles
This page shows how to Purify or remove serine proteases with a Benzamidine Sepharose 4 Fast Flow, Benzamidine Sepharose 4 Fast Flow from Cytiva.
This page provides information about the principles and standard conditions for different purification techniques for the purification of multiprotein complexes with from Cytiva.
This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from Cytiva.
This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.
Protocols
To protect the fusion protein from proteolytic degradation prior to enzymatic cleavage with PreScission Protease, thrombin, or Factor Xa, it may be necessary to remove proteases from the sample. Additionally, following enzymatic cleavage, it may be necessary to remove thrombin or Factor Xa from the sample.
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
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