GE17-5111-01

HiTrap^® IGY Purification

Cytiva 17-5111-01, pack of 1 × 5 mL

• UNSPSC Code: 23151816
• NACRES: NA.56

Properties

• ligand: 2-mercaptopyridine
• packaging: pack of 1 × 5 mL
• manufacturer/tradename: Cytiva 17-5111-01
• storage condition: (20% Ethanol)
• parameter: <20 mL/min flow rate; 42 psi
• bed size: 16 mm × 25 mm
• bed volume: 5 mL
• column I.D.: 16 mm
• matrix: highly cross-linked 6% agarose
• avg. part. size: 34 μm
• cleaning: 2-13
• working range: 3-11
• storage temp.: 2-30°C

Description

General description

HiTrap^® IgY Purification HP is a 5 mL column prepacked with Sepharose^™ High Performance for fast and easy purification of IgY from egg yolk.

Application

HiTrap^® IgY Purification HP is packed with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose^™ High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgY and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.

Features and Benefits

• Each column binds IgY from a one-quarter portion of egg yolk (approx. 100 mg IgY). For greater capacity, several columns may be connected in series.
• Packed with a thiophilic adsorption medium, the ligand, 2-merCaptopyridine is coupled to Sepharose^™ High Performance.
• Recovery and purity of IgY are excellent.
• Can be operated with a liquid chromatography system such as ÄKTA^® design.
• The top and bottom frits are manufactured from porous polyethylene
• Connectors for usage with different chromatography systems and other equipment are included

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Safety Information

• pictograms: GHS02
• signalword: Warning
• hcodes: H226
• pcodes: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class: 3 - Flammable liquids