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GE17-0709-01

DEAE Sepharose™ Fast Flow

Cytiva 17-0709-01, pack of 500 mL

Synonym(s):

DEAE Sepharose Resin

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

diethylaminoethyl

description

Ion Exchanger Type (value)

packaging

pack of 500 mL

manufacturer/tradename

Cytiva 17-0709-01

matrix

6% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning in place

2-14

working range

2-12

suitability

suitable for bioprocess medium

Related Categories

General description

DEAE Sepharose Fast Flow is a weak anion exchanger based on the well established Sepharose™ Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

DEAE Sepharose Fast Flow is part of the Sepharose™ Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. It is composed of crosslinked 6% agarose beads, with diethylaminoethyl (DEAE) weak anion exchange groups. DEAE Sepharose Fast Flow has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. Scale-up with DEAE Sepharose Fast Flow is straight forward and the medium is available in a range of pre-packed process development tools.

As member of the BioProcess media range, DEAE Sepharose Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

  • Well-proven weak anion exchanger developed for industrial downstream processes
  • Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals
  • The industry standard for ion exchange chromatography during recent decades
  • High chemical stability allows for well proven CIP and sanitization protocols
  • The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Danger

hcodes

H225

Hazard Classifications

Flam. Liq. 2

Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protocols

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service