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Key Documents

G0907

Sigma-Aldrich

Anti-Granulocyte-Macrophage Colony Stimulating Factor antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym(s):

GM-CSF, MGC131935, MGC138897, molgramostin, sargramostim

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About This Item

MDL number:
UNSPSC Code:
51111800
NACRES:
NA.41

biological source

goat

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

rat

technique(s)

western blot: 0.1 μg/mL

UniProt accession no.

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CSF2(1437)
mouse ... Csf2(12981)
rat ... Csf2(116630)

General description

GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils. GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity. It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines. The 22 kDa glycosylated GM-CSF, similar to IL-3 and IL-5, is a cytokine with a core of four bundled alpha -helices. Mature rat GM-CSF shares 56-69% amino acid sequence identity with canine, feline, human, mouse, and porcine GM-CSF. GM-CSF exerts its biological effects through a heterodimeric receptor complex composed of GM-CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high-affinity receptors for IL-3 and IL-5. In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF R alpha. Rat GM-CSF is active on mouse cells, although mouse GM-CSF is only weakly active on rat cells.

Specificity

Detects rat GM-CSF in direct ELISAs and Western blot. In direct ELISAs, approximately 100% cross-reactivity with recombinant feline GM-CSF and recombinant porcine GM-CSF is observed, approximately 30% cross-reactivity with recombinant mouse GM-CSF, recombinant canine GM-CSF and recombinant human GM-CSF is observed.

Immunogen

Purified recombinant rat GM-CSF expressed in E. coli.

Physical form

Lyophilized from a 0.2 μm filtered solution of phosphate buffered saline with 5% trehalose

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Andrew J Fleetwood et al.
Critical reviews in immunology, 25(5), 405-428 (2005-09-20)
GM-CSF was originally defined by its ability to generate in vitro granulocyte and macrophage colonies from bone marrow precursor cells. Apart from its physiological role in the control of alveolar macrophage development, it now appears more likely that its major
G G Wong et al.
Science (New York, N.Y.), 228(4701), 810-815 (1985-05-17)
Clones of complementary DNA encoding the human lymphokine known as granulocyte-macrophage colony-stimulating factor (GM-CSF) were isolated by means of a mammalian cell (monkey COS cell) expression screening system. One of these clones was used to produce recombinant GM-CSF in mammalian

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