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F7887

Sigma-Aldrich

Anti-Bovine IgG (whole molecule)–FITC antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Rabbit Anti-Bovine IgG (whole molecule)–Fluorescein isothiocyanate

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About This Item

MDL number:
MFCD00221531
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

storage condition

protect from light

technique(s)

direct immunofluorescence: 1:300
immunohistochemistry: 1:300

storage temp.

−20°C

target post-translational modification

unmodified

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General description

Immunoglobulin G (IgG) contributes to 10-20% of plasma protein and is regarded as one of the most predominant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50 kDa and 25 kDa, respectively.

Application

Anti-Bovine IgG (whole molecule)-FITC antibody produced in rabbit has been used in indirect fluorescent antibody test (IFAT).

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of FITC to Anti- Bovine IgG (whole molecule) antibody allows for the visualization of protein by fluorescent microscopy.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Larissa Alves et al.
Tropical animal health and production, 53(1), 76-76 (2021-01-07)
The aim of the present study was to evaluate the spatial distribution of the prevalence of T. gondii in cows using the indirect immunofluorescence assay and determine associated risk factors. Serum samples were collected from 2970 cows on 263 rural
Patricio M Calandra et al.
Revista Argentina de microbiologia, 46(4), 315-319 (2015-01-13)
The aim of this study was to describe two events in which Neospora caninum was involved in bovine abortions in beef cattle. In the first event, 11 abortions in 57 heifers were recorded in 45 days. One aborted heifer was
Gustavo Henrique Nogueira Costa et al.
Experimental parasitology, 127(1), 277-281 (2010-08-26)
Two studies, of a natural infection and an experimental infection, were performed in order to study congenital transmission of Toxoplasma gondii in cattle. In the first study, 50 fetuses were harvested from gestating cows that were eutanasied at a municipal
L D Monje et al.
Journal of medical entomology, 53(3), 660-665 (2016-01-23)
Several cases of human rickettsiosis caused by Rickettsia parkeri were recently documented in the Paraná River delta of Argentina, where the tick vector is Amblyomma triste Koch. As cattle suffer recurrent A. triste infestations, they are at risk of becoming
Serological survey of Neospora caninum and Toxoplasma gondii in cattle (Bos indicus) and water buffaloes (Bubalus bubalis) in ten provinces of Brazil
Spagnol FH, et al.
Revista Brasileira de Parasitologia veterinaria = Brazilian Journal of Veterinary Parasitology : Org?o Oficial do Colegio Brasileiro de Parasitologia Veterinaria, 52, 42-45 (2009)
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